Vaccination with recombinant whole heavy chain fragments of Clostridium botulinum type C and D neurotoxins
Vaccination with recombinant whole heavy chain fragments of Clostridium botulinum type C and D neurotoxins
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DOI：
10.1128/cdli.11.3.496-502.2004
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发表时间：
2004-05-01
期刊：
影响因子：
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通讯作者：
Oguma, K
中科院分区：
文献类型：
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作者：
Arimitsu, H;Lee, JC;Oguma, K
以表达为谷胱甘肽s转移酶融合蛋白的肉毒梭菌C型和D型神经毒素重组全重(H)链皮下免疫小鼠和鸭。在小鼠实验中，经免疫印迹分析和酶联免疫吸附试验证实，两次接种C型和D-H链（每次10杯），分别显著增加了针对C型和D型神经毒素100 kda H链的特异性抗体。用G型和D-H型链免疫的小鼠，在每只小鼠分别腹腔注射高达10(5)和10(6)最小致死剂量（MLD）的C-和d - 16s毒素时，没有表现出肉毒中毒症状。用100杯C-H型链免疫鸭。鸭还产生了针对C-H型链的特异性抗体，并对静脉注射103只鸭MLD c - 16s毒素的攻击表现出显著的保护作用。上述结果表明，重组全H链可作为家畜C型和D型肉毒杆菌中毒有效、安全的疫苗。
Mice and ducks were subcutaneously immunized with recombinant whole heavy (H) chains of Clostridium botulinum type C and D neurotoxins, which were expressed as glutathione S-transferase fusion proteins. In the case of mice, it was confirmed that two immunizations with type C- and D-H chains, 10 mug each time, significantly increased the specific antibodies against 100-kDa H chains of type C and D neurotoxins in an immunoblot analysis and an enzyme-linked immunosorbent assay, respectively. The mice immunized with type G and D-H chains showed no symptoms of botulism when they were challenged with C- and D-16 S toxins at doses, given intraperitoneally, of up to 10(5) and 10(6) minmum lethal doses (MLD), respectively, per mouse. Ducks were immunized with a total of 100 mug of type C-H chain. The ducks also developed specific antibodies to the type C-H chain and showed significant protection against a challenge with 103 duck MLD of C-16 S toxin given intravenously. These results indicate that recombinant whole H chains can be used as an effective and safe vaccine for type C and D botulism in domestic animals.