Diffusivity of Human Cartilage Endplates in Healthy and Degenerated Intervertebral Disks.
Diffusivity of Human Cartilage Endplates in Healthy and Degenerated Intervertebral Disks.
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健康和退化椎间盘中人体软骨终板的扩散性。
DOI：
10.1115/1.4056871
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Wu,Yongren
中科院分区：
文献类型：
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作者：
Ren,Pengling;Chen,Peng;Reeves,RussellA;Buchweitz,Nathan;Niu,Haijun;Gong,He;Mercuri,Jeremy;Reitman,CharlesA;Yao,Hai;Wu,Yongren
椎间盘上下表面的软骨终板（cep）是椎间盘和椎体之间主要的营养物质运输通道。被动扩散负责通过无血管的cep运输小的营养物质和代谢物分子。健康CEP的基线溶质弥散率先前已被研究过，然而与IVD变性相关的CEP弥散变化仍不清楚。本研究旨在利用光漂白后荧光恢复（FRAP）方法定量比较健康和退化的人cep中溶质扩散。从5例新鲜冷冻的人尸体脊柱和17例脊柱融合手术患者中分别收集了7例健康的cep和22例退化的cep。用FRAP法测定了荧光素钠在CEP径向和垂直方向上的扩散系数。通过测量平均x射线衰减来评估CEP钙化水平。在健康和退化的cep中，径向和轴向的溶质扩散系数没有差异。与健康cep相比，退化cep的平均溶质扩散率降低44%(健康：29.07μm2/s (CI: 23.96 ~ 33.62μm2/s)；退化:16.32μm2 / s (CI: 13.84 - -18.84μm2 / s), p < 0.001)。标准化x射线衰减值测定的平均溶质扩散系数与CEP钙化程度呈反比关系（ß = - 22.19, R2= 0.633, p < 0.001）。本研究提示CEP钙化明显阻碍了溶质通过椎间盘和椎体界面的扩散，为进一步了解IVD退变机制提供了线索。
The cartilage endplates (CEPs) on the superior and inferior surfaces of the intervertebral disk (IVD), are the primary nutrient transport pathways between the disk and the vertebral body. Passive diffusion is responsible for transporting small nutrient and metabolite molecules through the avascular CEPs. The baseline solute diffusivities in healthy CEPs have been previously studied, however alterations in CEP diffusion associated with IVD degeneration remain unclear. This study aimed to quantitatively compare the solute diffusion in healthy and degenerated human CEPs using a fluorescence recovery after photobleaching (FRAP) approach. Seven healthy CEPs and 22 degenerated CEPs were collected from five fresh-frozen human cadaveric spines and 17 patients undergoing spine fusion surgery, respectively. The sodium fluorescein diffusivities in CEP radial and vertical directions were measured using the FRAP method. The CEP calcification level was evaluated by measuring the average X-ray attenuation. No difference was found in solute diffusivities between radial and axial directions in healthy and degenerated CEPs. Compared to healthy CEPs, the average solute diffusivity was 44% lower in degenerated CEPs (Healthy: 29.07μm2/s (CI: 23.96–33.62μm2/s); degenerated: 16.32μm2/s (CI: 13.84–18.84μm2/s),p< 0.001). The average solute diffusivity had an inverse relationship with the degree of CEP calcification as determined by the normalized X-ray attenuation values (ß = −22.19, R2= 0.633; p < 0.001). This study suggests that solute diffusion through the disk and vertebral body interface is significantly hindered by CEP calcification, providing clues to help further understand the mechanism of IVD degeneration.