Comprehensive spectral endoscopy of topically applied SERS nanoparticles in the rat esophagus.
Comprehensive spectral endoscopy of topically applied SERS nanoparticles in the rat esophagus.
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DOI：
10.1364/boe.5.002883
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发表时间：
2014-09
影响因子：
3.4
通讯作者：
Y. Wang;Altaz Khan;S. Y. Leigh;Danni Wang;Ye Chen;D. Meza;Jonathan T. C. Liu
中科院分区：
文献类型：
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作者：
Y. Wang;Altaz Khan;S. Y. Leigh;Danni Wang;Ye Chen;D. Meza;Jonathan T. C. Liu
食管癌的早期检测和生物学研究将受益于先进的成像技术的发展，以筛选癌症发生之前和伴随的分子变化。表面增强拉曼散射（Sers）纳米颗粒（NPs）具有通过细胞表面生物标志物的敏感和多重表型分析来改善癌症检测和癌症进展的研究的潜力。在这里，一个微型内窥镜具有旋转扫描和轴向拉回已开发的2D光谱成像的Sers纳米粒子局部应用于管腔表面的大鼠食管。将来自低pM浓度的Sers NP混合物的拉曼信号以真实的时间解复用，以精确地计算NP的浓度和比率。离体和体内实验证明了沿着大鼠食管的整个长度局部应用和成像多路Sers纳米颗粒的可行性。
The early detection and biological investigation of esophageal cancer would benefit from the development of advanced imaging techniques to screen for the molecular changes that precede and accompany the onset of cancer. Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have the potential to improve cancer detection and the investigation of cancer progression through the sensitive and multiplexed phenotyping of cell-surface biomarkers. Here, a miniature endoscope featuring rotational scanning and axial pull back has been developed for 2D spectral imaging of SERS NPs topically applied on the lumenal surface of the rat esophagus. Raman signals from low-pM concentrations of SERS NP mixtures are demultiplexed in real time to accurately calculate the concentration and ratio of the NPs. Ex vivo and in vivo experiments demonstrate the feasibility of topical application and imaging of multiplexed SERS NPs along the entire length of the rat esophagus.