GLOMERULAR EPITHELIAL, MESANGIAL, AND ENDOTHELIAL-CELL LINES FROM TRANSGENIC MICE
GLOMERULAR EPITHELIAL, MESANGIAL, AND ENDOTHELIAL-CELL LINES FROM TRANSGENIC MICE
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DOI：
10.1038/ki.1988.53
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发表时间：
1988-03-01
影响因子：
19.6
通讯作者：
STRIKER, GE
中科院分区：
文献类型：
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作者：
MACKAY, K;STRIKER, LJ;STRIKER, GE
肾小球细胞的培养是了解单个肾小球细胞功能的重要工具。然而，肾小球的复杂性使得获得纯细胞群变得困难。培养肾小球内皮细胞也很困难，即使是混合细胞群。目前还没有任何来源的确定的肾小球细胞系。我们已经建立了克隆的肾小球上皮细胞、系膜细胞和内皮细胞的永久细胞系，这些细胞来自于一种对猿猴病毒40 （SV40）的早期区域进行转基因的小鼠系。这些小鼠在出生时看起来正常，但在三到四个月大的时候，它们的肾小球的硬化程度不同。尽管这些细胞的表型发生了改变，但它们仍保持着正常细胞的特征。这些细胞系可作为了解转基因小鼠肾小球硬化发病机制的有用工具，并可用于研究正常肾小球细胞不受表型改变的生物学特征。
The culture of glomerular cells has represented an important tool in the understanding of individual glomerular cell functions. However, the complexity of the glomerulus has made it difficult to obtain pure cell populations. It has also been difficult to culture glomerular endothelial cells, even as mixed cell populations. At present there are no established glomerular cell lines from any source. We have established permanent cell lines of cloned glomerular epithelial, mesangial, and endothelial cells from a line of mice transgenic for the early region of simian virus 40 (SV40). These mice appear normal at birth but by three to four months of age have sclerosis affecting a variable percentage of their glomeruli. The cells maintain features characteristic of their normal counterparts despite their transformed phenotype. These cell lines could be useful tools in understanding the pathogenesis of glomerulosclerosis in this transgenic mouse model and in studying those features of normal glomerular cell biology which are not altered by a transformed phenotype.