Attachment, spreading and growth in vitro of highly malignant and low malignant murine fibrosarcoma cells.
Attachment, spreading and growth in vitro of highly malignant and low malignant murine fibrosarcoma cells.
复制标题
高恶性和低恶性鼠纤维肉瘤细胞的体外附着、扩散和生长。
DOI：
10.1007/bf01758953
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发表时间：
1985
影响因子：
4
通讯作者：
McCoy,JP
中科院分区：
文献类型：
--
作者：
Varani,J;Grimstad,IA;Knibbs,RN;Hovig,T;McCoy,JP
从三种不同的甲基胆蒽诱导的小鼠纤维肉瘤中分离的高度恶性细胞系和低恶性细胞系，在无血清条件下和血清存在下检查其附着于塑料培养皿和胶原包被培养皿的能力。在所有条件下，来自三个高度恶性细胞系的大多数细胞粘附并扩散。到72小时，细胞数量显著增加，表明至少一些细胞已经经历了分裂（即使在不存在血清的情况下）。相比之下，在没有血清或存在0.1%血清的情况下，来自三个低恶性细胞系的细胞较少附着并扩散在塑料或胶原基质上。然而，当沿着低恶性细胞加入每皿15μg层粘连蛋白时，它们随后附着并扩散在塑料和胶原包被的皿上。先前的研究表明，高度恶性细胞系表达与层粘连蛋白交叉反应的细胞表面抗原，而低恶性细胞系则不表达。我们推测，高恶性和低恶性细胞之间的差异，在细胞表面层粘连蛋白样抗原的表达，有助于在附着和扩散能力的不同。这些差异也可能导致这些细胞恶性潜能的不同。
Highly malignant cell lines and low-malignant cell lines isolated from three different methylcholanthrene-induced murine fibrosarcomas were examined for their ability to attach to plastic dishes and collagen-coated dishes under serumfree conditions and in the presence of serum. Most of the cells from the three highly malignant lines attached and spread under all conditions. By 72h, there was a significant increase in the number of cells indicating that at least some of the cells had undergone division (even in the absence of serum). In contrast, fewer of the cells from the three low-malignant lines attached and spread on the plastic or collagen substrates in the absence of serum or in the presence of 0.1 per cent serum. However, when 15μg laminin per dish was added along with the lowmalignant cells, they then attached and spread on the plastic and collagen-coated dishes. Previous studies have indicated that the highly malignant lines express cell surface antigens that cross-react with laminin while the low-malignant cell lines do not. We speculate that the differences between the high- and low-malignant cells in the expression of cell surface laminin-like antigens contribute to the dissimilarities in attachment and spreading capacity. These differences may also contribute to the dissimilarity between these cells in malignant potential.