Immunotoxin in the Absence of P-Glycoprotein allows Selective Target Cell Killing in the Semi-Direct Antigen Presentation Pathway Following Organ Transplantation
Immunotoxin in the Absence of P-Glycoprotein allows Selective Target Cell Killing in the Semi-Direct Antigen Presentation Pathway Following Organ Transplantation
复制标题
缺乏 P-糖蛋白的免疫毒素可在器官移植后的半直接抗原呈递途径中选择性杀死靶细胞
DOI：
10.1097/01.tp.0000542614.79078.e4
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发表时间：
2018
期刊：
影响因子：
6.2
通讯作者：
Meader L
中科院分区：
文献类型：
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作者：
Meader L
结果经BrdU处理的BL/6 x CBA F1小鼠的肾脏移植到有或无IA k-gelonin的MDR-/-或野生型FVB受体中。移植后一天，收获脾脏用于免疫组织化学。供体BrdU+细胞可以容易地检测到。还对切片进行IA k表达染色，以定量BrdU+/IA k+（供体乘客白细胞）和BrdU-/IA k+细胞（获得供体MHC II类分子的受体细胞，即参与半直接抗原呈递的靶群体）。
ResultsKidneys from BL/6 x CBA F1 mice treated with BrdU were transplanted into MDR-/-, or wild type FVB, recipients with or without IA k-gelonin. One day after transplant the spleens were harvested for immunohistochemistry. Donor BrdU+ cells could be readily detected. Sections were also stained for IA k expression to quantify BrdU+/IA k+(donor passenger leukocytes) and BrdU-/IA k+ cells (recipient cells that have acquired donor MHC class II molecules, ie the target population that takes part in semi-direct antigen presentation).