Faster and easier chromatin immunoprecipitation assay with high sensitivity
Faster and easier chromatin immunoprecipitation assay with high sensitivity
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DOI：
10.1002/pmic.200600283
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发表时间：
2007-01-01
期刊：
影响因子：
3.4
通讯作者：
Murakami, Yota
中科院分区：
文献类型：
--
作者：
Kohzaki, Hidetsugu;Murakami, Yota
Chromatin immunoprecipitation (ChIP) assays are widely used to investigate where chromatin-binding proteins bind to the genome. The standard assay is very time consuming. We have developed a rapid ChIP assay in which the immunoprecipitates serve directly as PCR templates. This assay eliminates the step to reverse the crosslinking, shortening the assay by 1 day. It also requires a less immunoprecipitating antibody, permits many samples to be tested simultaneously, and is more sensitive than the standard ChIP assay.
