KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma.
KIAA1377 is associated with lymph node metastasis in esophageal squamous cell carcinoma.
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KIAA1377 与食管鳞状细胞癌的淋巴结转移相关。
DOI：
10.3892/ol.2016.5343
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发表时间：
2016
期刊：
影响因子：
2.9
通讯作者：
Xiaomei Lu
中科院分区：
文献类型：
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作者：
Shutao Zheng;Chenchen Yang;Qing Liu;Tao Liu;Mang Lu;Fang Dai;Xiang;I. Sheyhidin;Xiaomei Lu
KIAA1377在有淋巴结转移的食管鳞状细胞癌（ESCC）中较无淋巴结转移的食管鳞状细胞癌（ESCC）中显著扩增，但在细胞生物学和神经系统疾病方面的研究较少。提示KIAA1377可能在ESCC淋巴结转移中发挥作用。据我们所知，目前还没有研究调查KIAA1377在ESCC中的作用。本研究通过免疫组织化学检测KIAA1377的表达，并结合临床病理参数对其表达进行统计学分析，采用市购组织阵列，包括86例ESCC和79例配对对照。用特异性小发夹RNA （small hairpin RNA, shRNA）载体瞬时转染内源性KIAA1377水平最高的ESCC TE-1和EC9706细胞系，在体外敲除KIAA1377。分别用甲基噻唑四氮唑、创面愈合和Transwell法评价细胞增殖、迁移和侵袭的变化。在体内研究发现KIAA1377的表达与淋巴结转移和分化有显著相关性，在体外研究发现KIAA1377的下调对ESCC细胞系TE-1的增殖和移动性没有显著影响。总之，这是第一个表明KIAA1377可能在ESCC淋巴结微转移中发挥作用的研究。
KIAA1377, of which there are few studies regarding cell biology and neurological diseases, has been found to be significantly amplified in esophageal squamous cell carcinoma (ESCC) with lymph node metastasis compared with ESCC without lymph node metastasis. This suggests that KIAA1377 may play a role in the lymph node metastasis of ESCC. There has, to the best of our knowledge, been no study performed to investigate the role of KIAA1377 in ESCC. In the present study, the expression of KIAA1377 was detected by immunohistochemistry, and its expression was statistically analyzed with clinicopathological parameters, using commercially obtained tissue arrays consisting of 86 cases of ESCC and 79 paired controls. KIAA1377 was knocked down ex vivo using transient transfection with specific small hairpin RNA (shRNA) vectors into ESCC TE-1 and EC9706 cell lines whose endogenous KIAA1377 level was highest. The variation of proliferation, migration and invasion were evaluated using methyl thiazolyl tetrazolium, wound healing and Transwell assay, respectively. It was found in vivo that KIAA1377 expression was significantly associated with lymph node metastasis and differentiation, and ex vivo that knockdown of KIAA1377 cannot significantly affect proliferation and mobility in the ESCC cell line TE-1. Overall, this is the first study suggesting that KIAA1377 may play a role in the lymph node micrometastasis of ESCC.
影响因子：
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作者：
Cardiff, Robert D;Miller, Claramae H;Munn, Robert J
通讯作者：
Munn, Robert J