Smooth muscle cells produce an inhibitor of endothelial cell growth.
Smooth muscle cells produce an inhibitor of endothelial cell growth.
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平滑肌细胞产生内皮细胞生长抑制剂。
DOI：
10.1161/01.atv.5.1.110
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Eisenstein,R
中科院分区：
文献类型：
--
作者：
Schumacher,BL;Grant,D;Eisenstein,R
一种有效的内皮细胞生长和新血管形成抑制剂先前已从牛和人主动脉中分离出来。在试图确定该组织的主要细胞成分，平滑肌细胞，合成具有这种活性的分子，我们研究了牛主动脉平滑肌的无血清条件培养基对培养的牛主动脉内皮细胞生长的影响。平滑肌条件培养基被发现含有生长抑制和刺激活性，可以区分其热稳定性。该抑制剂可被硫酸铵沉淀，热稳定（80 ℃），并被二硫苏糖醇、胰蛋白酶和2-巯基乙醇灭活。它的估计分子量约为35，000至40，000道尔顿（通过柱色谱法）。因此，平滑肌细胞似乎产生一种内皮细胞生长抑制剂，该抑制剂属于一类源自无血管组织的天然内皮细胞生长抑制剂，我们暂时称为“avasculins”。“证明其与从完整主动脉（或其他组织）分离的抑制剂的同一性必须等待纯化。
A potent inhibitor of endothelial cell growth and neovascularization has previously been isolated from both bovine and human aorta. In an attempt to determine if the major cellular component of that tissue, smooth muscle cells, synthesized molecules with this activity, we studied the effects of serum-free conditioned medium from bovine aortic smooth muscle on the growth of cultured endothelial cells from bovine aorta. The smooth muscle conditioned medium was found to contain both growth inhibitory and stimulating activities that could be distinguished by their heat stability. The inhibitor is precipitable by ammonium sulfate, heat stable (80 degrees C), and inactivated by dithiothreitol, trypsin, and 2-mercaptoethanol. It has an estimated molecular weight of approximately 35,000 to 40,000 daltons (by column chromatography). It therefore appears that smooth muscle cells produce an inhibitor of endothelial cell growth that belongs to a class of natural endothelial cell growth inhibitors derived from avascular tissues we tentatively term "avasculins." Proof of its identity with the inhibitor isolated from intact aorta (or other tissues) must await purification.