AN EFFECT OF CODON CONTEXT ON THE MISTRANSLATION OF UGU CODONS INVITRO
AN EFFECT OF CODON CONTEXT ON THE MISTRANSLATION OF UGU CODONS INVITRO
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DOI：
10.1016/0022-2836(84)90443-1
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
BUCKINGHAM, RH
中科院分区：
文献类型：
--
作者：
CARRIER, MJ;BUCKINGHAM, RH
密码子环境对无意义密码子抑制的影响可能是通过释放因子识别终止密码子或核糖体选择氨基酰tRNA起作用的。通过比较大肠杆菌UGA抑制子色氨酸tRNA在两种合成聚合物PolyU(U-G)和Poly(U5，G)中对UGU(半胱氨酸)密码子的误读来研究后一种假设，这两种聚合物在UGU密码子周围的序列不同。将这些聚合物在无细胞体系中从大肠杆菌中体外翻译，产生了4倍的选择误差。10-3和1.75倍。Poly(U-G)和Poly(U5，G)中的UGU密码子分别为10-2。因此，密码子环境可能会显著影响蛋白质中氨基酸的错误结合。
Effects of codon context on nonsense codon suppression may act either through release factor recognition of termination codons or aminoacyl-tRNA selection by the ribosome. The latter hypothesis was studied by comparing misreading by Escherichia coli UGA suppressor tryptophan tRNA of UGU (cysteine) codons in 2 synthetic polymers, poly(U-G) and poly(U5,G), which differ in sequence around the UGU codons. In vitro translation of these polymers in a cell-free system from E. coli yielded selection errors of 4 .times. 10-3 and 1.75 .times. 10-2 for UGU codons in poly(U-G) and poly(U5,G), respectively. Thus, codon context may significantly affect misincorporation of amino acids into protein.