The phlogistic role of C3 leukotactic fragments in myocardial infarcts of rats.
The phlogistic role of C3 leukotactic fragments in myocardial infarcts of rats.
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DOI：
10.1084/jem.133.4.885
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发表时间：
1971-04-01
期刊：
影响因子：
--
通讯作者：
Ward PA
中科院分区：
文献类型：
--
作者：
Hill JH;Ward PA
描述了一种从大鼠心肌中提取酶的改进方法，该酶将补体的第三组分（C3）裂解成趋白细胞片段。使用放射性标记的人 C3 制剂已显示出 C3 的直接裂解。在凝胶过滤中，酶的洗脱稍微超出（延迟到）白蛋白标记。冠状动脉手术结扎后，可以从梗塞的大鼠心肌中提取白细胞趋化活性。根据其在梗塞组织中出现的时间来描述，趋白细胞活性已被鉴定为大鼠C3的裂解产物。 C3 片段分子量低且大小不均匀。如果用C3灭活剂（从眼镜蛇毒液中分离）处理大鼠以消除血清C3，则大鼠在梗塞心肌中不能产生白细胞趋化活性。反过来，在冠状动脉结扎后的前 48 小时内，几乎没有看到中性粒细胞因受感染的心肌而出现的通常强烈积聚。这些研究表明 C3 在介导非特异性组织损伤的急性炎症反应中具有非免疫学作用。
An improved method is described for extraction from rat myocardium of an enzyme that cleaves the third component of complement (C3) into leukotactic fragments. Direct cleavage of C3 has been shown by the use of a radiolabeled preparation of human C3. The enzyme elutes slightly beyond (retarded to) an albumin marker in gel filtration. After surgical ligation of a coronary artery, leukotactic activity can be extracted from infarcted rat myocardium. Described in terms of its time of appearance in infarcted tissue, the leukotactic activity has been identified as cleavage products of rat C3. The C3 fragments are of low molecular weight and heterogenous in size. Rats, if treated with the C3 inactivator (isolated from cobra venom) so as to ablate serum C3, fail to develop leukotactic activity in the infarcted myocardium. In turn, few if any of the usual intense accumulations of neutrophils in response to infected myocardium are seen during the first 48 hr after coronary artery ligation. These studies suggest a nonimmunologic role for C3 in the mediation of the acute inflammatory response in nonspecific tissue injury.