ORGANIZATION OF CONTRACTILE PROTEIN GENES WITHIN THE 88F-SUBDIVISION OF THE DROSOPHILA-MELANOGASTER 3RD CHROMOSOME
ORGANIZATION OF CONTRACTILE PROTEIN GENES WITHIN THE 88F-SUBDIVISION OF THE DROSOPHILA-MELANOGASTER 3RD CHROMOSOME
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DOI：
10.1016/0092-8674(84)90377-5
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
FYRBERG, EA
中科院分区：
文献类型：
--
作者：
KARLIK, CC;MAHAFFEY, JW;FYRBERG, EA
研究了黑腹龙第3染色体88F亚群内收缩蛋白基因的排列。至少有5种抗原性相关的肌原纤维蛋白，其中3种仅在间接飞行肌中积累，由位于act88F肌动蛋白基因近140千碱基处的20千碱基染色体片段编码。染色体片段包括2个转录区，每个转录区指导多种mRNA的合成。RNA印迹杂交实验和DNA测序表明，重叠转录物是由差异RNA剪接产生的。核苷酸序列还确定了2个编码蛋白是原肌球蛋白亚型，2个与原肌球蛋白相关。肌原纤维基因的排列与破坏间接飞行肌形成的第三染色体突变的分布相似。
Contractile protein gene arrangement was investigated within the 88F subdivision of the D. melanogaster 3rd chromosome. At least 5 antigenically related myofibrillar proteins, 3 of which accumulate only within indirect flight muscles, are encoded by a 20 kilobase chromosome segment located 140 kilobases proximal to the act88F actin gene. The chromosome segment includes 2 transcribed regions, each of which directs the synthesis of multiple mRNA. RNA blot-hybridization experiments and DNA sequencing suggest that overlapping transcripts are generated by differential RNA splicing. The nucleotide sequence also establishes that 2 of the encoded proteins are tropomyosin isoforms, and 2 re related to tropomyosin. The arrangement of myofibrillar genes parallels the distribution of 3rd chromosome mutations that disrupt indirect flight muscle formation.