Characterization of penicillin intermediate serotypes of Streptococcus pneumoniae carried by human immunodeficiency virus-infected adults and healthy children in Uganda
Characterization of penicillin intermediate serotypes of Streptococcus pneumoniae carried by human immunodeficiency virus-infected adults and healthy children in Uganda
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DOI：
10.1089/mdr.2006.9993
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发表时间：
2007-03-01
影响因子：
2.6
通讯作者：
Jacobs, M. R.
中科院分区：
文献类型：
--
作者：
Blossom, D. B.;Cordeiro, S. M.;Jacobs, M. R.
关于乌干达人群中的耐抗生素肺炎球菌分离株之间的遗传相关性的数据很少。从 1995 年从乌干达儿童和 2004-2005 年感染人类免疫缺陷病毒 (HIV) 的乌干达成年人中分离出的代表 26 个荚膜血清群或血清型的 166 个分离株中，选出了以前很少或没有报道过的青霉素中间型肺炎球菌。通过脉冲场凝胶电泳 (PFGE) 和多位点序列分型 (MLST) 进一步表征了两个患者群体中存在的相同血清群或血清型的青霉素中间型肺炎球菌对。发现了七对这样的分离株，包括血清组 7、11、15B/C 和 16 以及血清型 13、21 和 35B。这七对分离株的 PFGE 未显示血清群或血清型之间存在克隆性，仅在血清群 11 和血清型 13 内存在克隆性。14 个分离株的 MLST 揭示了 13 种不同的序列类型 (ST)，其中 11 种以前未曾记录。与所有已知 ST 的比较表明，大多数这些菌株仅与其他国家的相同血清型菌株相关，这些相关菌株通常也是青霉素中间体。这些发现表明，乌干达对青霉素不敏感的原因可能是由于抗生素耐药性肺炎球菌克隆被引入乌干达，而不是该国境内出现了耐药性。
There are little data on the genetic relatedness between antibiotic-resistant pneumococcal isolates colonizing the Ugandan population. Penicillin-intermediate pneumococci of serogroups or serotypes rarely or not previously reported as being penicillin nonsusceptible were selected out of 166 isolates representing 26 capsular serogroups or serotypes isolated from Ugandan children in 1995 and human immunodeficiency virus (HIV)infected Ugandan adults in 2004-2005. Pairs of penicillin-intermediate pneumococci of the same serogroup or serotype present in both patient populations were characterized further by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Seven such pairs of isolates were found and included serogroups 7, 11, 15B/C, and 16 as well as serotypes 13, 21, and 35B. PFGE of these seven pairs showed no clonality between serogroups or serotypes, and clonality only within serogroup 11 and serotype 13. MLST of the 14 individual isolates revealed 13 different sequence types (STs), 11 of which had not previously been recorded. Comparisons with all known STs revealed that most of these strains were related only to strains of the same serotype in other countries, with these related strains frequently also being penicillin intermediate. These findings suggest that penicillin nonsusceptibility in Uganda is likely due to the introduction of antibiotic-resistant pneumococcal clones into Uganda rather than development of resistance within the country.