Influenza A virus hemagglutinin protein subunit vaccine elicits vaccine-associated enhanced respiratory disease in pigs
Influenza A virus hemagglutinin protein subunit vaccine elicits vaccine-associated enhanced respiratory disease in pigs
复制标题
DOI：
10.1016/j.vaccine.2014.07.059
复制
发表时间：
2014-09-08
期刊：
影响因子：
5.5
通讯作者：
Vincent, Amy L.
中科院分区：
文献类型：
--
作者：
Rajao, Daniela S.;Loving, Crystal L.;Vincent, Amy L.
当猪在存在由全灭活病毒（WIV）疫苗引发的非中和但交叉反应性抗体的情况下用异源病毒攻毒时，可能发生疫苗相关增强型呼吸道疾病（VAERD）。本研究旨在比较猪接种2009年H1N1流感病毒（H1N1 pdm 09）重组血凝素（HA）亚单位疫苗（HA-SV）或温度敏感性减毒活流感病毒（LAW）疫苗后，对异源Δ 1 -H1 N2甲型流感病毒（IAV）攻毒的效果，并评估HA免疫在VAERD发生中的作用。HA-SV和LAIV疫苗均诱导了针对具有同源HA的病毒（H1N1 pdm 09）的高中和抗体，但不诱导针对异源攻击病毒（delta 1-H1 N2）的高中和抗体。LAW部分保护猪，导致病毒脱落减少和病毒清除更快，因为在感染后5天（dpi）在肺中未检测到病毒。HA-SV接种猪在攻毒后出现更严重的肺和气管病变，与VAERD一致。这些结果表明，针对HA蛋白的单独免疫应答足以在异源攻击后引起VAERD。爱思唯尔有限公司出版
Vaccine-associated enhanced respiratory disease (VAERD) can occur when pigs are challenged with heterologous virus in the presence of non-neutralizing but cross-reactive antibodies elicited by whole inactivated virus (WIV) vaccine. The aim of this study was to compare the effects of heterologous delta 1 -H1N2 influenza A virus (IAV) challenge of pigs after vaccination with 2009 pandemic H1N1 virus (H1N1pdm09) recombinant hemagglutinin (HA) subunit vaccine (HA-SV) or temperature-sensitive live attenuated influenza virus (LAW) vaccine, and to assess the role of immunity to HA in the development of VAERD. Both HA-SV and LAIV vaccines induced high neutralizing antibodies to virus with homologous HA (H1N1pdm09), but not heterologous challenge virus (delta 1-H1N2). LAW partially protected pigs, resulting in reduced virus shedding and faster viral clearance, as no virus was detected in the lungs by 5 days post infection (dpi). HA-SV vaccinated pigs developed more severe lung and tracheal lesions consistent with VAERD following challenge. These results demonstrate that the immune response against the HA protein alone is sufficient to cause VAERD following heterologous challenge. Published by Elsevier Ltd.