Effects of Dietary Zinc-Methionine Supplementation During Pregnancy on the Whole-Genome Methylation and Related Gene Expression in the Liver and Spleen of Growing Goats: a Short Communication
Effects of Dietary Zinc-Methionine Supplementation During Pregnancy on the Whole-Genome Methylation and Related Gene Expression in the Liver and Spleen of Growing Goats: a Short Communication
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妊娠期日粮补充蛋氨酸锌对生长山羊肝脏和脾脏全基因组甲基化及相关基因表达的影响：简短交流
DOI：
10.1007/s12011-020-02223-7
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发表时间：
2020-06-01
影响因子：
3.9
通讯作者：
Tan, Zhiliang
中科院分区：
文献类型：
--
作者：
Li, Qiushuang;Yan, Qiongxian;Tan, Zhiliang
研究了用有机锌替代妊娠山羊日粮中无机锌对仔羊肝脏和脾脏发育的影响。选择胎次相近、体重相近（BW 37.17 ± 5.28 kg）的湘东黑山羊妊娠期山羊14只，随机分为硫酸锌组（ZnSO_4组，n = 7）和蛋氨酸锌组（Zn-Met组，n = 7）。山羊喂养45天（妊娠第106天至分娩）。哺乳期山羊分娩后，饲喂不额外补充锌的日粮。小山羊在2月龄断奶，两组饲喂相同的日粮。所有山羊均饲喂混合饲料，并可自由饮用淡水。100日龄时屠宰公山羊，取肝脏和脾脏称重，液氮保存，进行基因组DNA甲基化和相关基因表达测定。蛋氨酸锌组的肝器官指数呈上升趋势（P < 0.10），但对肝脏和脾脏的全基因组甲基化没有影响（P > 0.10）。仔鼠血锌含量降低（P < 0.05），肝、脾甲基化相关基因表达下调（P < 0.05）或呈下降趋势（P < 0.10）。这些数据表明，山羊在妊娠期间饲喂蛋氨酸锌可增加后代肝脏器官指数，而不会改变基因组DNA甲基化。推测血锌对锌指蛋白Sp3的调节，间接调节了小山羊肝脏和脾脏中甲基化相关基因的表达，从而增强了后代免疫系统的发育和功能。
The effect of replacing inorganic zinc with organic zinc in diets of pregnant goats was investigated on the development of the liver and spleen of offspring. Pregnant goats (n = 14; Xiangdong black goat, local breed) of similar parity and body weight (BW, 37.17 +/- 5.28 kg) were selected and divided randomly into two groups: the zinc sulfate group (ZnSO4;n = 7) and the methionine-chelated zinc group (Zn-Met;n = 7). Goats were fed for 45 days (day 106 of gestation to delivery). After delivering, lactating goats were fed a diet without extra zinc supplement. Kid goats were weaned at 2 months of age and both the groups were fed the same diet. All goats were fed a mixed diet and had free access to fresh water. Kid goats were slaughtered on day 100, and the liver and spleen were collected, weighed, and stored in liquid nitrogen for genomic DNA methylation and related gene expression determination. In the Zn-Met group, the liver organ index of kid goats showed an increasing trend (P < 0.10), but the methylation of the whole genome was not affected both in the liver and spleen (P > 0.10). Furthermore, the blood zinc content of the offspring was reduced (P < 0.05), and the expression of genes related to methylation were downregulated (P < 0.05) or showed a downward trend (P < 0.10) in the liver and spleen. These data indicated that goats feeding Zn-Met during pregnancy increased the offspring liver organ index without change in the genomic DNA methylation. It is speculated that the regulation of zinc finger protein Sp3 adjusted by blood zinc indirectly regulated the expression of methylation-related genes in the liver and spleen of the kid goats, thus enhancing the development and function of the immune system of the offspring.