Mathematical analysis of DNA distributions derived from flow microfluorometry.
Mathematical analysis of DNA distributions derived from flow microfluorometry.
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DOI：
10.1083/jcb.60.2.523
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发表时间：
1974-02
期刊：
影响因子：
--
通讯作者：
Jett JH
中科院分区：
文献类型：
--
作者：
Dean PN;Jett JH
流动微荧光（FMF）技术的发展（Van Dilla等人，1969；Kraemer等人，1972）用于以高速率（bbb20 000/分钟）测量单细胞DNA含量，从而实现了以前无法获得的统计精度和灵敏度。它开辟了新的方法，一般细胞表征和生命周期分析，特别是。本文提出了一种基于计算机的数学技术，用于从DNA分布的FMF光谱中计算GI， S和G2+ M分数。通过[3H]-胸腺嘧啶放射自显影证实了指数生长细胞S部分的结果。
The development of flow microfluorornetric (FMF) techniques (Van Dilla et al., 1969; Kraemer et al., 1972) for the measurement of DNA content of single cells at high rates (> 20,000/min) has permitted a statistical precision and sensitivity not obtainable previously. It has opened up new approaches to cell characterization in general and to life cycle analysis in particular. This paper presents a computer-based mathematical technique for calculating GI, S, and G2+ M fractions from FMF spectra of DNA distributions. Results for the S fraction of exponentially growing cells are confirmed by [3H]-thymidine autoradiography.