Photoreactive, core-shell cross-linked/hollow microspheres prepared by delayed addition of cross-linker in dispersion polymerization for antifouling and immobilization of protein.
Photoreactive, core-shell cross-linked/hollow microspheres prepared by delayed addition of cross-linker in dispersion polymerization for antifouling and immobilization of protein.
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DOI：
10.1016/j.jcis.2012.08.047
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发表时间：
2013
影响因子：
9.9
通讯作者：
Shengliu Wang;Kai Yue;Lianying Liu;Wantai Yang
中科院分区：
文献类型：
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作者：
Shengliu Wang;Kai Yue;Lianying Liu;Wantai Yang
当苯乙烯(St)的分散聚合反应进行3h后，在颗粒快速生长阶段后，在反应体系中加入4，4‘-二甲基丙烯酰氧基二苯甲酮(DMABP)交联剂，制得表面富含二苯甲酮(BP)基团的核(PST)-壳(PST-co-DMABP)粒子。DMABP的聚合主要发生在PST的预制核上，因为(1)交联剂加入时形成的粒子致密(80%以上的单体已被消耗掉，粒子不再被单体完全溶胀)，(2)连续相极性降低，以及(3)立即发生交联，从而阻碍了DMABP的扩散。随后，通过去除THF中的未交联核，得到了具有光反应活性的交联型中空粒子。扫描电子显微镜和透射电子显微镜观察表明，随着DMABP含量的增加，核壳结构的形成和壳层厚度的增加。对溶解在四氢呋喃中的聚合物进行了UV-Vis光谱分析，结果表明岩心中不存在DMABP聚合物。FTIR光谱分析和XPS测试进一步表明，随着DMABP用量的增加，粒子表面的BP组分更加丰富。最后，通过红外光谱分析、扫描电子显微镜观察和TMB显色反应，在紫外光照射下，将小鼠免疫球蛋白和聚乙二醇防污聚合物固定在粒子表面。
When dispersion polymerization of styrene (St) had run for 3h, after particle rapidly growing stage, 4,4′-dimethacryloyloxybenzophenone (DMABP) cross-linker was added to reaction system and photoreactive, core(PSt)–shell(Poly(St-co-DMABP)) particles with rich benzophenone (BP) groups on surface were prepared. Polymerization of DMABP could occurred mainly on the preformed core of PSt because its diffusion could be impeded by (1) compactness of particles formed at the moment of cross-linker addition (more than 80% of monomer had been consumed, particles were no longer fully swollen by monomer), (2) reduced polarity of continuous phase, and (3) immediate occurrence of cross-linking. Subsequently, photoreactive, cross-linked hollow particles were yielded by removal of uncross-linked core in THF. SEM and TEM observation demonstrated the formation of core–shell structure and improvement of shell thickness when DMABP content increased. UV–vis spectra analysis on polymer dissolved in THF indicated that there is no polymer of DMABP in core. FTIR spectra analysis and XPS measurement further revealed that BP component on particle surface was enriched when amount of DMABP increased. Finally, an anti-fouling polymer (poly (ethylene glycol), PEG) and protein of mouse IgG was immobilized on particle surface under UV irradiation, as confirmed by FTIR spectra analysis, SEM observation and TMB color reaction.