Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro
Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro
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DOI：
10.1007/s13277-014-2826-8
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发表时间：
2015-03-01
期刊：
影响因子：
--
通讯作者：
Liu, Chang
中科院分区：
文献类型：
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作者：
Zhang, Jingyao;Wu, Qifei;Liu, Chang
Sld five 1（PSF 1）的伴侣是称为GINS的异四聚体复合物的成员。已有研究表明，PSF 1在多种肿瘤中表达不稳定，与肿瘤的恶性程度有关。然而，PSF 1在肺癌中的作用仍不清楚。本研究的目的是探讨PSF 1对肺癌细胞增殖能力的影响。首先，采用实时荧光定量RT-PCR和Western blotting方法检测22例肺癌组织及癌旁组织中PSF 1的表达。我们的研究结果表明，与邻近的非肿瘤样本相比，PSF 1在肺癌样本中过表达。为了更好地了解PSF 1在肺癌细胞中的功能，我们使用PSF 1特异性小干扰RNA（siRNA）成功地在mRNA和蛋白水平抑制PSF 1的表达。此外，我们使用了具有不同p53基因背景（p53无效和p53野生型）的肺癌细胞系。结果表明，PSF 1基因敲低抑制肺癌细胞的增殖，并引起细胞周期阻滞的p53非依赖性的方式。我们的数据表明，PSF 1在功能上参与肺癌细胞增殖，是肺癌治疗的潜在靶点。
Partner of sld five 1 (PSF1) is a member of the heterotetrameric complex termed GINS. Previous studies have shown that PSF1 is unregulated in several cancer and associated with tumor malignant characters. However, the effects of PSF1 in lung cancer are still unclear. The goal of this study was to investigate the effects of PSF1 on the proliferation capacities of lung cancer. To start with, expression of PSF1 in 22 human lung cancer samples and adjacent non-tumor samples were detected by real-time RT-PCR and Western blotting. Our results showed that PSF1 was overexpressed in lung cancer samples compared to adjacent non-tumor samples. To achieve better insights of PSF1 functions in lung cancer cells, we used PSF1-specific small interfering RNA (siRNA) successfully inhibit the expression of PSF1 in messenger RNA (mRNA) and protein levels. In addition, we used lung cancer cell lines with different p53 gene background (p53 null and p53 wild-type). The results showed that knockdown of PSF1 inhibited cell proliferation and caused cell cycle arrest of lung cancer cells in a p53-independent manner. Our data indicated that PSF1 is functionally involved in lung cancer cell proliferation and is a potential target for lung cancer therapy.