In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions
In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions
复制标题
体外粒细胞粘附和体内边缘化：两种相关的补体依赖性功能
DOI：
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发表时间：
1977
影响因子：
15.3
通讯作者：
H. S. Jacob
中科院分区：
文献类型：
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作者：
Jorg Fehr;Jorg Fehr;H. S. Jacob
为了研究血管内粒细胞在循环池和边缘池之间分布的调节机制和介质，我们分析了一个具有极端短暂边缘的人体模型——连续流滤过性白细胞电泳中性粒细胞减少症。动物研究证实了补体(C)衍生的粒细胞减少诱导因子的存在。因此，自体血浆暴露于过滤系统的尼龙纤维（NF）中，产生循环粒细胞和单核细胞的急性选择性减少。这一现象可通过血浆失补、EDTA或肼预处理以及56℃预热来阻止，但在NF暴露前将热灭活和肼处理的血浆重新组合后确实会发生。在50℃下预热血浆不抑制中性粒细胞减少反应，提示参与了C活化的经典途径。超滤研究表明，nf诱导的中性粒细胞减少诱导因子的摩尔重量在10,000-30,000之间，并且热稳定（56℃）。为了分析C诱导的中性粒细胞边缘可能是由于细胞对内皮表面的粘附性增加的假设，我们在体外评估了C在促进粒细胞粘附方面的作用。用塑料培养皿法测定，在热灭活（56℃）和肼处理的血浆中，粒细胞粘附性显著降低，但通过混合两种血浆或在肼处理的血浆中加入纯化的C3，粘附能力得以恢复。暴露于活化的C后，中性粒细胞表现出显著增加的粘附性，当细胞在热灭活的血浆中重悬时，这种粘附性保持不变，但在新鲜血浆中重悬时，这种粘附性逐渐丧失。基于这些结果，我们得出结论，粒细胞在体外的粘附性和在体内的边缘是密切相关的，c依赖现象。
To study mechanisms and mediators regulating the distribution of intravascular granulocytes between circulating and marginated pools, a human model with extreme transient margination, the neutropenia of continuous flow filtration leukophoresis, was analyzed. Studies in animals demonstrated the existence of a complement (C)-derived granulocytopenia-inducing factor. Thus, autologous plasma, exposed to nylon fibers (NF) of the filtration system, produced an acute selective decrement of circulating granulocytes and monocytes. This phenomenon was blocked by decomplementing plasma, by pretreatment of plasma with EDTA or hydrazine, and by preheating at 56 degrees C, but did occur after recombination of heat-inactivated and hydrazine-treated plasma before NF exposure. Preheating plasma at 50 degrees C did not inhibit the neutropenic response, suggesting involvement of the classical pathway of C activation. Ultrafiltration studies indicated that the NF-provoked neutropenia-inducing factor has a mol wt in the range of 10,000-30,000, and is heat stable (56 degrees C). To analyze the hypothesis that C- induced neutrophil margination might be consequent to increased cell adhesiveness to endothelial surfaces, the role of C in promoting granulocyte adherence was evaluated in vitro. Measured with a plastic Petridish assay, granulocyte adherence was significantly reduced in heat- inactivated (56 degrees C) and hydrazine-treated plasma, but adherence promoting capacity was restored by mixing the two plasmas, or by adding purified C3 to hydrazine-treated plasma. After exposure to activated C, neutrophils showed significantly increased adhesiveness which was maintained when cells were resuspended in heat-inactivated plasma, but progressively lost when resuspended in fresh plasma. On the basis of these results we conclude that granulocyte adhesiveness in vitro and margination in vivo are closely associated, C-dependent phenomena.