Lipid accumulation and growth characteristics of Chlorella zofingiensis under different nitrate and phosphate concentrations
Lipid accumulation and growth characteristics of Chlorella zofingiensis under different nitrate and phosphate concentrations
复制标题
不同硝酸盐和磷酸盐浓度下佐芬小球藻的脂质积累和生长特性。
DOI：
10.1016/j.jbiosc.2012.05.007
复制
发表时间：
2012-10-01
影响因子：
2.8
通讯作者：
Hu, Zhengyu
中科院分区：
文献类型：
--
作者：
Feng, Pingzhong;Deng, Zhongyang;Hu, Zhengyu
为了评价绿色小球藻作为生物柴油原料的潜力，研究了氮和磷对小球藻脂肪积累和生长的影响。当NaNO_3和K_2HPO的浓度分别为1.0g L~(-1)和0.01g L~(-1)时，最大比生长速率(m(Max))达到2.15d~(-1)。缺氮(65.1%)和缺磷(44.7%)培养基细胞的脂肪含量均高于全培养细胞(33.5%)。缺氮培养细胞的产脂率最高(87.1mg·L~(-1)d~(-1))，说明缺氮比缺磷更有利于诱导细胞内脂肪的积累。此外，还测试了在60个L平板光生物反应器和10个L瓶户外培养生物柴油的可行性。结果表明，条斑扇贝能较好地适应室外温度和光照的变化，最高单位单位产量可达0.362天(-1)，最高产脂能力可达26.6mg·L-1天(-1)。在室外养殖条件下，其产脂潜力为31.1 kg ha(-1)d(-1)。这些结果表明，华支睾吸虫是一种很有前途的生物燃料原料生产菌种，可以在室外进行放大培养。(C)2012年，日本生物技术学会。版权所有。
To evaluate the potential of the green agla Chlorella zofingiensis as a feedstock for biodiesel production, the effects of nitrogen and phosphate on lipid accumulation and growth of C. zofingiensis were studied. The maximum specific growth rate (mu(max)) reached 2.15 day(-1) when the concentration of NaNO3 and K2HPO4 center dot 3H(2)O was 1.0 g L-1 and 0.01 g L-1 respectively. The lipid contents of C. zofingiensis grown in media deficient of nitrogen (65.1%) or phosphate (44.7%) were both higher than that obtained from cells grown in full medium (33.5%). The highest lipid productivity (87.1 mg L-1 day(-1)) was also obtained from cells grown in nitrogen deficient media, indicating nitrogen deficiency was more effective than phosphate deficiency for inducing lipid accumulation in C. zofingiensis. In addition, the feasibility of cultivating the alga in 60 L flat plate photobioreactors and 10 L bottles outdoors for biodiesel was also tested. It was found that C. zofingiensis could adapt to fluctuating temperatures and irradiance of outdoors and the highest mu(max) and lipid productivity could reach 0.362 day(-1) and 26.6 mg L-1 day(-1) outdoors, respectively. The lipid production potential of C. zofingiensis is projected to be 31.1 kg ha(-1) day(-1) in outdoor culture. These results suggested that C. zofingiensis is a promising organism for feedstock production of biofuel and can be used in scaled up culture outdoors. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.