Real-time in vivo virtual histology of colorectal lesions when using the endocytoscopy system
Real-time in vivo virtual histology of colorectal lesions when using the endocytoscopy system
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DOI：
10.1016/j.gie.2006.01.021
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发表时间：
2006-06-01
影响因子：
7.7
通讯作者：
Shiokawa, Akira
中科院分区：
文献类型：
--
作者：
Sasajima, Keita;Kudo, Shin-ei;Shiokawa, Akira
背景：胃肠道病变的组织学表现是基于H& E染色的薄片标本的光学显微镜检查。最近，提出了光学活检的概念。在内窥镜检查过程中，使用细胞内镜观察结直肠病变以获得实时组织学图像的研究进行了。设计：前瞻性研究。目的：评估使用细胞内镜（E-C）系统进行结直肠病变光学活检的有用性。患者：受试者为113名连续患者，他们从2003年4月至2004年3月接受了完整的结肠检查，由一名结肠镜医师进行检查。结果：使用E-C，可以在细胞水平上观察病变，并在体内评价除结构病变外的细胞病变。内镜诊断与组织学诊断的相关性有统计学意义。局限性：内镜必须接触到靶结肠腺体。结论：内镜可以区分肿瘤性与非肿瘤性病变，也可以区分浸润性癌与腺瘤。“超高”放大内镜，即E-C系统，提供实时体内组织学图像，与H& E染色显微镜图像吻合良好。
Background: The histological findings of GI lesions are based on light-microscopic examination of H&E-stained thin-slice specimens. Recently, a concept of optical biopsy has been advocated. A study of the observation of colorectal lesions using endocytoscopy to obtain real-time histological images in vivo during endoscopy was performed.Design: Prospective study.Aim: To evaluate the usefulness of optical biopsy of colorectal lesions with the endocytoscopy (E-C) system.Patients: The subjects were 113 consecutive patients who underwent a complete colonic examination, from April 2003 to March 2004, performed by a single colonoscopist.Setting: Digestive Disease Center of Showa University Northern Yokohama Hospital.Results: With E-C, it was possible to observe lesions at the cellular level and evaluate cellular atypia in addition to structural atypia in vivo. The correlation was statistically significant between the endocytoscopic diagnosis and the histological diagnosis.Limitations: The endocytoscope had to be touched to the target colonic glands.Conclusions: It was possible to distinguish neoplastic from non-neoplastic lesions, and also possible to distinguish invasive cancer from adenoma. 'Ultra-high' magnifying endoscopy, the E-C system, provides real-time histological images in vivo, which correspond well with those of H&E-stained microscopic images.