Evolution of buffering in a genetic circuit controlling plant stem cell proliferation
Evolution of buffering in a genetic circuit controlling plant stem cell proliferation
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DOI：
10.1038/s41588-019-0389-8
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发表时间：
2019-05-01
期刊：
影响因子：
30.8
通讯作者：
Lippman, Zachary B.
中科院分区：
文献类型：
--
作者：
Rodriguez-Leal, Daniel;Xu, Cao;Lippman, Zachary B.
精确控制植物干细胞的增殖对于植物器官的持续和可复制发育是必要的(1，2)。多肽配体CLAVATA3(CLV3)及其受体蛋白激酶CLAVATA1(CLV1)在一个高度保守的负反馈回路(1，2)内维持干细胞的动态平衡。在拟南芥中，CLV1同源基因也有助于动态平衡，通过转录上调补偿CLV1的丢失(3)。在这里，我们表明，补偿(4，5)在不同的谱系中对配体和受体都起作用，但虽然核心的CLV信号模块是保守的，补偿机制却是多样化的。配位体之间的转录补偿在番茄中发挥作用，这是由于一个古老的基因复制影响了果实大小的驯化。相反，我们在拟南芥和玉米中几乎没有发现配体之间存在转录补偿的证据，而番茄和拟南芥之间的受体补偿也不同。我们的发现表明，配体和受体类似物之间的补偿对干细胞的稳态至关重要，但不同的遗传机制缓冲了保守的发育程序。
Precise control of plant stem cell proliferation is necessary for the continuous and reproducible development of plant organs(1,2). The peptide ligand CLAVATA3 (CLV3) and its receptor protein kinase CLAVATA1 (CLV1) maintain stem cell homeostasis within a deeply conserved negative feedback circuit(1,2). In Arabidopsis, CLV1 paralogs also contribute to homeostasis,bycompensatingforthe loss of CLV1through transcriptional upregulation(3). Here, we show that compensation(4,5) operates in diverse lineages for both ligands and receptors, but while the core CLV signaling module is conserved, compensation mechanisms have diversified. Transcriptional compensation between ligand paralogs operates in tomato, facilitated by an ancient gene duplication that impacted the domestication of fruit size. In contrast, we found little evidence for transcriptional compensation between ligands in Arabidopsis and maize, and receptor compensation differs between tomato and Arabidopsis. Our findings show that compensation among ligand and receptor paralogs is critical for stem cell homeostasis, but that diverse genetic mechanisms buffer conserved developmental programs.