Effects of Ciji Hua'ai Baosheng granule formula (CHBGF) on life time, pathology, peripheral blood cells of tumor chemotherapy model mouse with H22 hepatoma carcinoma cells.
Effects of Ciji Hua'ai Baosheng granule formula (CHBGF) on life time, pathology, peripheral blood cells of tumor chemotherapy model mouse with H22 hepatoma carcinoma cells.
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DOI：
10.4314/ajtcam.v11i4.16
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发表时间：
2014-05
期刊：
影响因子：
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通讯作者：
Shengyan Xi;Rongjian Hong;Jingru Huang;Dawei Lu;Linchao Qian;Pengcheng Li;Lei Wen;Yanhui Wang
中科院分区：
文献类型：
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作者：
Shengyan Xi;Rongjian Hong;Jingru Huang;Dawei Lu;Linchao Qian;Pengcheng Li;Lei Wen;Yanhui Wang
背景技术慈济化癌保生颗粒方（CHBGF）是一种传统的中药经验方，可以帮助接受化疗的肿瘤患者对抗毒素和副作用，从而改善和延长生命。本研究旨在从整体水平评价CHBGF对H22肝癌细胞皮下移植瘤或腹水瘤化疗模型小鼠生存时间、肿瘤组织病理学和改善外周血细胞的生活质量的改善作用。材料与方法92只昆明种小鼠中71只右腋前皮下注射H22肝癌细胞，7天后已形成肿瘤，腹腔注射Cytoxan（CTX）（200mg/kg）建立小鼠移植瘤化疗模型，按随机数字表法分为8组。 21只小鼠腹腔注射CTX，同样方法建立模型。评价对生存时间影响的组分别为模型组、CHBGF组和阳性对照组，每组7只。抗癌效果评价组为模型组、三个治疗组和阳性对照组，每组10只。生存时间观察组分别给予生理盐水灌胃、CHBGF（64g/kg）每日1次，5-氟尿嘧啶（25mg/kg）腹腔注射隔日1次。观察各组存活时间。 5个抗癌观察组分别给予生理盐水灌胃、CHBGF（64g/kg、32g/kg、16g/kg）每日1次，5-氟尿嘧啶（25mg/kg）腹腔注射隔日1次。治疗21天后，移植的肿瘤被剥离。通过刺眼球采集血液并通过血液分析仪进行分析。并计算化疗后移植瘤抑制率。光镜下观察肿瘤组织及血涂片的病理变化。结果CHBGF（64g/kg）组移植瘤的寿命延长率为20.14%，且生存时间长于5-氟尿嘧啶组（P<0.05）。 CHBGF(64g/kg)腹水瘤组的寿命延长率为64.15%，生存时间长于模型组(P<0.01)和5-氟尿嘧啶组(P<0.05)。模型组移植瘤生长速度快于CHBGF(64g/kg)组和5-氟尿嘧啶组(P<0.05)。阳性药物组和CHBGF(64g/kg、32g/kg)组肿瘤平均重量较模型组轻(P<0.05或P<0.01)。 CHBGF（64g/kg、32g/kg、16g/kg）组的抑制率分别为31.15%、21.31%、13.11%。光镜下观察，阳性药物组和3个CHBGF组肿瘤组织病理恶化程度较模型组轻，CHBGF组WBC分布较模型组和5-氟尿嘧啶组明显。 CHBGF（64g/kg、32g/kg、16g/kg）组WBC和PLT下降幅度小于模型组和5-氟尿嘧啶组（P<0.05或P<0.01），仅CHBGF（64g/kg）组RBC和HGB数量多于模型组和5-氟尿嘧啶组（P<0.05）。结论慈济化爱保生颗粒方能延长H22肝癌细胞皮下移植瘤和腹水瘤化疗模型小鼠的生存时间，对皮下移植瘤化疗后的生长有一定的抑制作用，对化疗引起的WBC和PLT下降有一定的治疗作用。
BACKGROUND Ciji Hua'ai Baosheng Granule Formula (CHBGF) is a traditional Chinese empirical formula that can help the tumor patients who have received chemotherapy antagonize the toxin and side-effects so as to improve and prolong the life. This study is to evaluate the effects of CHBGF on improving life quality in terms of survival time, pathology of tumor tissue and ameliorating peripheral blood cells in mouse chemotherapy model with subcutaneous transplanted tumor or ascitic tumor of H22 hepatoma carcinoma cells at an overall level. MATERIALS AND METHODS 71 mice among the 92 Kunming mice were injected subcutaneously into the right anterior armpit with H22 hepatoma carcinoma cells, after 7 days, which had formed tumors and were used peritoneal injection of Cytoxan (CTX) (200mg/kg) to establish the mouse chemotherapy model with transplanted tumor, and then which were commensurately divided into 8 groups by random digits table. 21 mice were injected into peritoneal cavity to use CTX and the same method to establish the model. The groups for evaluating the effects on the survival time were the model, CHBGF and positive control group respectively with 7 mice in each group. The groups for evaluating the effects on anti-cancer were the model group, three treatment groups and positive control group with 10 mice in each group. The survival-time-observing groups were given intragastric administration of normal saline, CHBGF (64g/kg) once a day, and peritoneal injection of 5-Fluorouracil (25mg/kg) once every other day respectively. The survival time of each group was observed. The five anti-cancer-observing groups were given intragastric administration of normal saline, CHBGF (64g/kg, 32g/kg and 16g/kg) once a day, and peritoneal injection of 5-Fluorouracil (25mg/kg) once every other day respectively. After treatment for 21 days, the transplanted tumors were peeled off. Blood was collected through pricking eyeball and analyzed by hematology analyzer. And postchemotherapy transplanted tumor inhibition ratios were calculated. Pathological changes of tumor tissues and blood smears were observed with light microscope. RESULTS The life prolonging rate of CHBGF (64g/kg) group with transplanted tumor is 20.14%, and their survival time was longer than that of the 5-Fluorouracil group (P<0.05). Life prolonging rate of CHBGF (64g/kg) group with ascitic tumor is 64.15%, the survival time was longer than that of the model group (P<0.01) and the 5-Fluorouracil group (P<0.05). The growth of the transplanted tumor in model group was faster than that in CHBGF (64g/kg) group and 5-Fluorouracil group (P<0.05). The tumor average weight of the positive drug and the CHBGF (64g/kg, 32g/kg) groups was lighter than that of the model group (P<0.05 or P<0.01). The inhibition ratios of CHBGF (64g/kg, 32g/kg and 16g/kg) groups are 31.15%, 21.31%, and 13.11% respectively. Under light microscope, in the positive drug and three CHBGF groups the pathological deteriorated severity of tumor tissue observed was milder than that in the model group, the distribution of WBC in CHBGF groups was more obvious than that of the model and 5-Fluorouracil groups. The WBC and PLT decrease in CHBGF (64g/kg, 32g/kg and 16g/kg) groups is less than the model and the 5-Fluorouracil group (P<0.05 or P<0.01), the number of RBC and HGB just in the CHBGF (64g/kg) group was more than that of the model group or the 5-Fluorouracil group (P<0.05). CONCLUSION Ciji Hua'ai Baosheng Granule Formula can prolong the survival time of the mice chemotherapy model of both subcutaneous transplanted tumor and ascitic tumor of H22 hepatoma carcinoma cells, has some determinate inhibitory effects on the growth of subcutaneous transplanted tumor chemo-treated, and has the therapeutic effect on antagonizing decrease of WBC and PLT caused by chemotherapy.