A plant-derived edible vaccine against hepatitis B virus
A plant-derived edible vaccine against hepatitis B virus
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DOI：
10.1096/fasebj.13.13.1796
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发表时间：
1999-10-01
期刊：
影响因子：
4.8
通讯作者：
Legocki, AB
中科院分区：
文献类型：
--
作者：
Kapusta, J;Modelska, A;Legocki, AB
传染性肝炎B病毒代表42 nm球形双壳颗粒。然而，对来自B型肝炎病毒携带者的血液的分析揭示了由病毒包膜表面蛋白组成的较小的22 nm颗粒的存在。这些颗粒是高度免疫原性的，并已被用于酵母生产的B型肝炎病毒疫苗的设计。在酵母中表达后，这些蛋白质形成用于肠胃外免疫的病毒样颗粒。因此，将编码B型肝炎病毒表面抗原的DNA片段导入农杆菌LBA4404中，获得转基因羽扇豆（Lupinus luteus L.）和莴苣（LactucasativaL.）CV. Burpee Bibb表达包膜表面蛋白。喂食转基因羽扇豆组织的小鼠产生了显著水平的B型肝炎病毒特异性抗体。人类志愿者，用表达B型肝炎病毒表面抗原的转基因莴苣植物喂养，产生了对植物产生的蛋白质的特异性血清IgG应答。
The infectious hepatitis B virus represents 42 nm spherical double-shelled particles. However, analysis of blood from hepatitis B virus carriers revealed the presence of smaller 22 nm particles consisting of a viral envelope surface protein. These particles are highly immunogenic and have been used in the design of hepatitis B virus vaccine produced in yeast. Upon expression in yeast, these proteins form virus-like particles that are used for parenteral immunization. Therefore, the DNA fragment encoding hepatitis B virus surface antigen was introduced into Agrobacterium tumerifacience LBA4404 and used to obtain transgenic lupin (Lupinus luteus L.) and lettuce (Lactuca sativa L.) cv. Burpee Bibb expressing envelope surface protein. Mice that were fed the transgenic lupin tissue developed significant levels of hepatitis B virus-specific antibodies. Human volunteers, fed with transgenic lettuce plants expressing hepatitis B virus surface antigen, developed specific serum-IgG response to plant produced protein.