An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy
An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy
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原位光照射固态核磁共振光谱揭示了一种活性光感受器中间体
DOI：
10.1016/j.bpj.2011.10.022
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Tomonaga;Y.;Hidaka;T;Kawamura;I;Nishio;T;Ohsawa;K;Okitsu;T;Wada;A;Sudo;Y;Kamo;N;Ramamoorthy;A;Naito;A.
据我们所知，一种用于固态核磁共振测量的新型原位光辐射系统得到了改进，并被证明能够成功识别出嵌入在模型膜中的pharaonisphoborhodopsin （ppR或sensory rhodopsin II）的m -光中间体、带有传感器的复合物（ppR/pHtrII）和T204A突变体。[20-13C]retinal-ppR和ppr /pHtrII的13c核磁共振信号显示，在连续光照射条件下，多个具有13-cis， 15-anti - retinal构型的m -中间体共存。从光活化视网膜观察到的核磁共振信号提供了对ppr /pHtrII复合体光循环过程的见解。
A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate ofpharaonisphoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The13C NMR signals from [20-13C]retinal-ppR andppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-antiretinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in theppR/pHtrII complex.