Nuclear magnetic resonance-determined lipoprotein abnormalities in nonhuman primates with the metabolic syndrome and type 2 diabetes mellitus
Nuclear magnetic resonance-determined lipoprotein abnormalities in nonhuman primates with the metabolic syndrome and type 2 diabetes mellitus
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DOI：
10.1016/j.metabol.2007.01.015
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发表时间：
2007-06-01
影响因子：
9.8
通讯作者：
Hansen, Barbara C.
中科院分区：
文献类型：
--
作者：
Ding, Shi-Ying;Tigno, Xenia T.;Hansen, Barbara C.
代谢综合征（MetS）和2型糖尿病（T2 DM）患者的血脂谱通常以甘油三酯水平升高和高密度脂蛋白（HDL）胆固醇水平降低为特征。然而，在每种脂蛋白类别中，变化更为复杂。本研究使用核磁共振（NMR）光谱以及经典的β-定量方法，明确了非人灵长类动物中血脂异常的特征，并检查了与MetS确定的主要因素相关的多种脂蛋白组分的模式。将73只恒河猴分为3组：健康猴、MetS猴和T2 DM猴。血脂异常的特征。代谢综合征和2型糖尿病组的高脂血症包括富含磷脂酰肌醇的极低密度脂蛋白、中密度脂蛋白和小而致密的低密度脂蛋白（LDL）颗粒水平升高。还观察到大LDL和大HDL颗粒的浓度降低以及LDL和HDL颗粒尺寸减小。相关性分析显示，血糖和血脂谱差，葡萄糖耐受不良，胰岛素抵抗与致动脉粥样硬化的核磁共振谱。与传统的脂质组相比，NMR脂蛋白谱更详细地呈现了MetS和糖尿病血脂异常之间的显著差异，并证明了这两组之间脂蛋白类内颗粒大小和数量的显著和不同的变化。详细的脂蛋白谱分析可能为更及时的干预提供额外的指标。恒河猴很可能为新药试验提供一个很好的模型，旨在解决这3组中脂蛋白组分谱的特定差异，这些差异反映了从正常到显性糖尿病的病理生理学进展。(c)2007年爱思唯尔公司All rights reserved.
The lipid profile in patients with the metabolic syndrome (MetS) and type 2 diabetes mellitus (T2DM) is commonly characterized by increased levels of triglycerides; and decreased levels of high-density lipoprotein (HDL) cholesterol. However, within each lipoprotein class, the changes arc more complex. The present study clefined the characteristics of dyslipidernia among nonhuman primates, using nuclear magnetic resonance (NMR) spectroscopy as well as the classic beta-quantification method, and examined the pattern of multiple lipoprotein fractions in relation to the main factors identified with the MetS. Seventy-three rhesus monkeys were classified into 3 groups: healthy monkeys, monkeys with MetS, and monkeys with T2DM. Characteristics of dyslipidemia. in the MetS and T2DM groups included increased levels of triglyceride-rich very low-density lipoprotein, intermediate-density lipoprotein, and small, dense, low-density lipoprotein (LDL) particles. Reduced concentrations of large LDL and large HDL particles together with reduction of LDL and HDL particle sizes were also observed. Correlation analysis revealed that poor glycemic and lipid profiles, glucose intolerance, and insulin resistance were associated with an atherogenic NMR profile. Compared with the conventional lipid panel, the NMR lipoprotein profile presented in greater detail distinctive differences between the dyslipidemia of the MetS and that of diabetes and demonstrated significant and divergent shifts in both particle size and number within lipoprotein classes between those 2 groups. Detailed lipoprotein profiling may provide additional indicators for more timely intervention. Rhesus monkeys are likely to provide an excellent model for novel drug testing designed to address the specific differences in lipoprotein fraction profile across these 3 groups that reflect the progression of pathophysiology from normal to overt diabetes. (c) 2007 Elsevier Inc. All rights reserved.