Binding the tobacco mosaic virus to inorganic surfaces
Binding the tobacco mosaic virus to inorganic surfaces
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DOI：
10.1021/la035425o
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发表时间：
2004-01-20
期刊：
影响因子：
3.9
通讯作者：
Kern, K
中科院分区：
文献类型：
--
作者：
Knez, M;Sumser, MP;Kern, K
研究了烟草花叶病毒（TMV）在金属和绝缘体表面的吸附行为和表面化学。TMV作为一个管状的超分子模型系统与精确已知的表面终止。我们表明，如果衬底的表面化学和分子表面的pH依赖性化学相匹配，例如，通过氢键，发生强吸附，并且横向运动受到阻碍。由于病毒粒子的固定，可以通过原子力显微镜（AFM）以接触模式成像。我们还使用具有酰氯基团的自组装单层通过酯的形成来诱导共价键合。非接触式AFM证明，TMV保持其圆柱形的横截面只有在弱吸附条件下，也就是说，在疏水表面上，而在亲水性基板上的变形发生相互作用的化学基团的数量最大化。
We studied the adsorption behavior and surface chemistry of the tobacco mosaic virus (TMV) on well-defined metal and insulator surfaces. TMV serves as a tubular supramolecular model system with precisely known surface termination. We show that if the surface chemistry of the substrate and the pH-dependent chemistry of the molecular surface match, for example, by hydrogen bonding, a strong adsorption occurs, and lateral movement is impeded. Due to the immobilization, the virion can be imaged by atomic force microscopy (AFM) in contact mode. We also used self-assembled monolayers with an acyl chloride group to induce covalent bonding via ester formation. Noncontact AFM proved that TMV keeps its cylindrical cross section only under weak adsorption conditions, that is, on hydrophobic surfaces, while on hydrophilic substrates a deformation occurs to maximize the number of interacting chemical groups.