miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells
miR-142-5p Improves Neural Differentiation and Proliferation of Adipose-Derived Stem Cells
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miR-142-5p 改善脂肪干细胞的神经分化和增殖
DOI：
10.1159/000495054
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
王玮
中科院分区：
文献类型：
--
作者：
王玮
背景/目的：miRNAs可调控脂肪干细胞（ADSCs）的神经分化。在本研究中，我们假设miR-142- 5 p可以抑制RhoA/ROCK 1通路在ADSCs神经发生中的表达。方法：鉴定ADSCs向神经源性分化过程中表达失调的miRNA。免疫组化和western blot检测神经元特异性烯醇化酶（NSE）和β III微管蛋白（Neuron-specific class III beta-tubulin）的表达，作为神经元分化的标志。通过双荧光素酶测定、qRT-PCR和western blot验证miR-142- 5 p对RhoA和ROCK 1的靶向作用。miR-142- 5 p和RhoA/ROCK 1信号通路的作用通过使用功能实验（包括细胞活力和集落形成测定）来探索。结果：miR-142- 5 p在ADSCs神经源性分化过程中表达显著上调。内源性miR-142- 5 p的敲低阻碍了神经源性分化。MiR-142- 5 p可直接靶向RhoA和ROCK 1 mRNA，抑制其表达，从而增加NSE和β III微管蛋白阳性的分化细胞比例。RhoA/ROCK 1信号通路参与miR-142- 5 p对ADSCs神经源性分化的影响。结论：我们的研究结果表明，miR-142- 5 p作为一种促生长的miRNA发挥作用，并通过靶向脂肪干细胞中的RhoA/ROCK 1在神经源性分化中发挥重要作用。
Background/Aims: MiRNAs may regulate neurogenic differentiation of adipose-derived stem cells (ADSCs). In this study, we hypothesized that the miR-142-5p can repress the expression of RhoA/ROCK1 pathway on the neurogenesis of ADSCs. Methods: Deregulated miRNA during neurogenic differentiation of ADSCs were identified. The expression of neuron-specific enolase (NSE) and β III tubulin (Neuron-specific class III beta-tubulin) were detected as the markers of neurogenic differentiation by immunostaining and western blot. The targeting of miR-142-5p on RhoA and ROCK1 was verified by dual luciferase assay, qRT-PCR and western blot. The roles of miR-142-5p and the RhoA/ROCK1 signaling pathway were explored by using functional experiments including cell viability and colony formation assays. Results: MiR-142-5p is significantly upregulated during neurogenic differentiation of ADSCs. Knockdown of endogenous miR-142-5p hampered neurogenic differentiation. MiR-142-5p could directly target RhoA and ROCK1 mRNA and repress their expressions, through which it increased the proportion of differentiated cells with positive NSE and β III tubulin. RhoA/ROCK1 signaling pathway is involved in miR-142-5p effect on the process of neurogenic differentiation of ADSCs. Conclusion: Our results demonstrate that miR-142-5p functions as a growth promotive miRNA and plays an important role in neurogenic differentiation by targeting RhoA/ROCK1 in ADSCs.
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影响因子：
3.7
作者：
Matsumoto Y;Inden M;Tamura A;Hatano R;Tsukita S;Asano S
通讯作者：
Asano S
影响因子：
9
作者：
通讯作者：
--
影响因子：
4.1
作者：
Li, Jianwei;Hu, Chen;Long, Jie
通讯作者：
Long, Jie
影响因子：
5.6
作者：
Kim, Yeon Jeong;Hwang, Soo Hyun;Jung, Jin Sup
通讯作者：
Jung, Jin Sup
DOI：
10.1016/j.diff.2015.10.010
发表时间：
2015-11
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Ho Park;Hyeon Park;Ha-Jin Pak;D. Yang;Yun-Hong Kim;Won-Jun Choi;Se-Jin Park;Jung-Ah Cho;
通讯作者：
Ho Park;Hyeon Park;Ha-Jin Pak;D. Yang;Yun-Hong Kim;Won-Jun Choi;Se-Jin Park;Jung-Ah Cho;