Resident cellular components of the human lung: current knowledge and goals for research on cell phenotyping and function.
Resident cellular components of the human lung: current knowledge and goals for research on cell phenotyping and function.
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DOI：
10.1513/pats.200803-025hr
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发表时间：
2008-09-15
期刊：
影响因子：
--
通讯作者：
Williams, Mary C
中科院分区：
文献类型：
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作者：
Franks, Teri J;Colby, Thomas V;Williams, Mary C
该研讨会的目的是确定仍然模糊或新的肺细胞成分，以确定肺发育和健康中影响疾病的细胞功能，并为未来的研究确定有希望的途径，以提取和表型这些细胞。由于强大的技术现在可用于识别，分类，纯化，培养和表型细胞，现在可以看到进展，以揭示发育阶段和疾病中肺细胞的起源和功能能力。研讨会的议程是首先讨论肺的胚胎发育，包括祖细胞和干细胞，然后评估肺的三个主要部分的功能和结构细胞：（1）支气管和细支气管上皮和支气管腺体中的气道细胞（基底细胞、分泌细胞、纤毛细胞、克拉拉细胞和神经内分泌细胞）;（2）肺泡单位细胞（1型细胞、2型细胞和肺内成纤维细胞）;和（3）肺血管细胞（来自不同血管结构的内皮细胞、平滑肌细胞和外膜成纤维细胞）。主要建议是：（1）用更好的细胞标志物（表面和非表面）表征肺内的各种细胞，包括祖细胞和干细胞;（2）获得更多关于健康和疾病中特定细胞类型的基因表达的知识，这将为生物学和病理学过程提供见解;（3）开发更多的细胞培养、分离、分选、共培养和永生化方法;以及（4）促进组织库，以便于从正常和患病的肺中获取组织，用于各级分析。
The purpose of the workshop was to identify still obscure or novel cellular components of the lung, to determine cell function in lung development and in health that impacts on disease, and to decide promising avenues for future research to extract and phenotype these cells. Since robust technologies are now available to identify, sort, purify, culture, and phenotype cells, progress is now within sight to unravel the origins and functional capabilities of lung cells in developmental stages and in disease. The Workshop's agenda was to first discuss the lung's embryologic development, including progenitor and stem cells, and then assess the functional and structural cells in three main compartments of the lung: (1) airway cells in bronchial and bronchiolar epithelium and bronchial glands (basal, secretory, ciliated, Clara, and neuroendocrine cells); (2) alveolar unit cells (Type 1 cells, Type 2 cells, and fibroblasts in the interstitium); and (3) pulmonary vascular cells (endothelial cells from different vascular structures, smooth muscle cells, and adventitial fibroblasts). The main recommendations were to: (1) characterize with better cell markers, both surface and nonsurface, the various cells within the lung, including progenitor cells and stem cells; (2) obtain more knowledge about gene expression in specific cell types in health and disease, which will provide insights into biological and pathologic processes; (3) develop more methodologies for cell culture, isolation, sorting, co-culture, and immortalization; and (4) promote tissue banks to facilitate the procurement of tissue from normal and from diseased lung for analysis at all levels.