Advances in fluorescence imaging with quantum dot bio-probes
Advances in fluorescence imaging with quantum dot bio-probes
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DOI：
10.1016/j.biomaterials.2005.11.018
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Weiss, S
中科院分区：
文献类型：
--
作者：
Pinaud, F;Michalet, X;Weiss, S
经过几个小组在表面化学开发和优化方面的大量努力，荧光半导体纳米晶体探测器，也被称为量子点或Q点，现在正在进入生物应用领域，为生物学家提供了很多东西。通往成功的道路上铺满了障碍，但从这些努力中产生了许多原始的表面化学物质，生物领域的科学家可以从这些化学物质中获得具体的生物应用。通过使用一种或多种最近开发的量子点涂层来轻松地调节量子点表面的化学性质的能力，以及它们特殊的光物理特性，一直是量子点获得革命性荧光生物探针地位的关键因素。确实是这样。量子点的独特性质不仅使生物学家有机会探索先进的成像技术，如单分子或终身成像，而且还可以重新审视传统的荧光成像方法，在体外或体内提取尚未观察到的或无法获取的信息。(C)2005爱思唯尔有限公司。保留所有权利。
After much effort in surface chemistry development and optimization by several groups, fluorescent semiconductor nanocrystals probes, also known as quantum dots or qdots, are now entering the realm of biological applications with much to offer to biologists. The road to success has been paved with hurdles but from these efforts has stemmed a multitude of original surface chemistries that scientists in the biological fields can draw from for their specific biological applications. The ability to easily modulate the chemical nature of qdot surfaces by employing one or more of the recently developed qdot coatings, together with their exceptional photophysics have been key elements for qdots to acquire a status of revolutionary fluorescent bio-probes. Indeed. the unique properties of qdots not only give biologists the opportunity to explore advanced imaging techniques such as single molecule or lifetime imaging but also to revisit traditional fluorescence imaging methodologies and extract yet unobserved or inaccessible information in vitro or in vivo. (C) 2005 Elsevier Ltd. All rights reserved.