CIRCADIAN CLOCK FUNCTIONS LOCALIZED IN XENOPUS RETINAL PHOTORECEPTORS
CIRCADIAN CLOCK FUNCTIONS LOCALIZED IN XENOPUS RETINAL PHOTORECEPTORS
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DOI：
10.1016/0896-6273(93)90160-s
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发表时间：
1993-04-01
期刊：
影响因子：
16.2
通讯作者：
BESHARSE, JC
中科院分区：
文献类型：
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作者：
CAHILL, GM;BESHARSE, JC
调节视觉功能的昼夜节律振荡器位于脊椎动物眼睛内的某处。为了确定昼夜节律是否由视网膜感光细胞产生，我们从爪蟾视网膜中分离并培养了感光层。平均而言，这些制剂中 94% 的活细胞是视杆细胞或视锥细胞光感受器。在持续黑暗的环境下，感光层有节奏地产生褪黑激素，平均周期为 24.3 小时。通过在体外将光感受器层暴露于光或喹吡罗（一种 D2 多巴胺受体激动剂）循环中，褪黑激素节律的相位被重置。这些数据表明眼睛的其他部分对于视网膜昼夜褪黑激素节律的产生或夹带不是必需的，并且表明视杆细胞和/或视锥细胞光感受器是昼夜节律时钟细胞。
A circadian oscillator that regulates visual function is located somewhere within the vertebrate eye. To determine whether circadian rhythmicity is generated by retinal photoreceptors, we isolated and cultured photoreceptor layers from Xenopus retina. On average, 94% of the viable cells in these preparations were rod or cone photoreceptors. Photoreceptor layers produced melatonin rhythmically, with an average period of 24.3 hr, in constant darkness. The phase of the melatonin rhythm was reset by in vitro exposure of the photoreceptor layers to cycles of either light or quinpirole, a D2 dopamine receptor agonist. These data indicate that other parts of the eye are not necessary for generation or entrainment of retinal circadian melatonin rhythms and suggest that rod and/or cone photoreceptors are circadian clock cells.