Ferritin acts as the most abundant binding protein for snowdrop lectin in the midgut of rice brown planthoppers (Nilaparvata lugens)
Ferritin acts as the most abundant binding protein for snowdrop lectin in the midgut of rice brown planthoppers (Nilaparvata lugens)
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DOI：
10.1016/s0965-1748(99)00130-7
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发表时间：
2000-04-01
影响因子：
3.8
通讯作者：
Gatehouse, JA
中科院分区：
文献类型：
--
作者：
Du, JP;Foissac, X;Gatehouse, JA
甘露糖特异性雪花莲凝集素[雪花莲凝集素（GNA）]显示对水稻褐飞虱的毒性。一种26 kDa的GNA结合多肽，来自N.通过凝集素印迹和亲和层析鉴定褐飞虱中肠，并通过N-末端测序进行表征。该多肽是N.褐飞虱中肠从N. lugens cDNA文库推导的氨基酸序列与烟草天蛾及其他节肢动物、植物和脊椎动物的铁蛋白亚基具有显著的同源性，并含有一个推测的N-糖基化位点。天然铁蛋白是从整个昆虫中纯化出来的一种大小超过400 kDa的蛋白质，并进行了生物化学表征。从天然复合物中释放出20、26和27 kDa的三个亚基。26 kDa亚基结合GNA，其N-末端序列与fersub 2相同。第二个cDNA（fersub 1）与双翅目昆虫铁蛋白具有很强的同源性，被鉴定为在一个N. lugens中肠特异性cDNA文库，并能编码较大的铁蛋白亚基。fersub 1 cDNA在起始密码子上游携带茎环结构（铁响应元件），类似于已显示在其他昆虫中控制铁蛋白合成中发挥作用的结构。(C)2000爱思唯尔科技有限公司版权所有。
The mannose-specific snowdrop lectin [Galanthus nivalis agglutinin (GNA)] displays toxicity to the rice brown planthopper Nilaparvata lugens. A 26 kDa GNA-binding polypeptide from N. lugens midgut was identified by lectin blotting and affinity chromatography, and characterized by N-terminal sequencing. This polypeptide is the most abundant binding protein for GNA in the N. lugens midgut. A cDNA (fersub2) encoding this protein was isolated from an N. lugens cDNA library. The deduced amino acid sequence shows significant homology to ferritin subunits from Manduca sexta and other arthropods, plants and vertebrates, and contains a putative N-glycosylation site. Native ferritin was purified from whole insects as a protein of more than 400 kDa in size and characterized biochemically. Three subunits of 20, 26 and 27 kDa were released from the native complex. The 26 kDa subunit binds GNA, and its N-terminal sequence was identical to that of fersub2. A second cDNA (fersub1), exhibiting strong homology with dipteran ferritin, was identified as an abundant cDNA in an N. lugens midgut-specific cDNA library, and could encode the larger ferritin subunit. The fersub1 cDNA carries a stem-loop structure (iron-responsive element) upstream from the start codon, similar to structures that have been shown to play a role in the control of ferritin synthesis in other insects. (C) 2000 Elsevier Science Ltd. All rights reserved.