Peroxisomes exhibit compromised structure and matrix protein content in SARS-CoV-2-infected cells.
Peroxisomes exhibit compromised structure and matrix protein content in SARS-CoV-2-infected cells.
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DOI：
10.1091/mbc.e21-02-0074
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发表时间：
2021-07-01
影响因子：
3.3
通讯作者：
Rachubinski RA
中科院分区：
文献类型：
--
作者：
Knoblach B;Ishida R;Hobman TC;Rachubinski RA
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种新型冠状病毒，引发了全球健康和经济危机。在这里，我们报告的影响SARS-CoV-2感染对人类细胞株Huh-7和SK-N-SH的过氧化物酶体。过氧化物酶体在SARS-CoV-2感染的细胞中发生显著的形态变化。过氧化物酶体膜重排后，过氧化物酶体基质蛋白重新分布到胞质溶胶中，导致成熟过氧化物酶体数量急剧减少。SARS-CoV-2 ORF 14蛋白被证明与人PEX 14物理相互作用，PEX 14是基质蛋白输入和过氧化物酶体生物合成所需的过氧化物酶体膜蛋白。鉴于过氧化物酶体在先天免疫中的重要作用，SARS-CoV-2可能直接靶向过氧化物酶体，导致过氧化物酶体结构完整性、基质蛋白含量和抗病毒信号传导功能的丧失。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that has triggered global health and economic crises. Here we report the effects of SARS-CoV-2 infection on peroxisomes of human cell lines Huh-7 and SK-N-SH. Peroxisomes undergo dramatic changes in morphology in SARS-CoV-2-infected cells. Rearrangement of peroxisomal membranes is followed by redistribution of peroxisomal matrix proteins to the cytosol, resulting in a dramatic decrease in the number of mature peroxisomes. The SARS-CoV-2 ORF14 protein was shown to interact physically with human PEX14, a peroxisomal membrane protein required for matrix protein import and peroxisome biogenesis. Given the important roles of peroxisomes in innate immunity, SARS-CoV-2 may directly target peroxisomes, resulting in loss of peroxisome structural integrity, matrix protein content and ability to function in antiviral signaling.