Determination of serum IgG antibodies to Bacillus anthracis protective antigen in environmental sampling workers using a fluorescent covalent microsphere immunoassay.
Determination of serum IgG antibodies to Bacillus anthracis protective antigen in environmental sampling workers using a fluorescent covalent microsphere immunoassay.
复制标题
使用荧光共价微球免疫分析法测定环境采样人员中炭疽杆菌保护性抗原的血清 IgG 抗体。
DOI：
10.1136/oem.2003.008565
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
MacKenzie,BA
中科院分区：
文献类型：
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作者：
Biagini,RE;Sammons,DL;Smith,JP;Page,EH;Snawder,JE;Striley,CAF;MacKenzie,BA
目的：评估炭疽恐怖袭击后采样/净化工作人员接触炭疽芽孢杆菌 (Ba) 孢子的可能性。方法：2001 年 10 月发生炭疽袭击后，从参与佛罗里达州博卡拉顿市美国媒体公司 (AMI) 大楼的芽孢杆菌环境采样的工作人员那里采集了 56 份血清样本。其中 19 份血清样本是从入境前的个人中采集的。以及进入大楼几周后。在进入前和后续抽血时，各从独特的个体中抽取了九份血清。还评估了十三份供体对照血清。使用新开发的荧光共价微球免疫测定 (FCMIA)，通过测量血清 Ba 抗保护性抗原 (PA) 特异性 IgG 抗体来调查个体的 Ba 暴露情况。结果：四种血清给出阳性抗 PA IgG 结果（定义为抗 PA IgG 浓度⩾ 供体对照血清中的平均 μg/ml 抗 PA IgG（n = 13 加 2 SD，也受到抑制⩾）阳性血清是 2000 年接受最后一剂炭疽疫苗的两名工作人员的入境前和随访样本。结论：本研究的采样/净化工作人员要么没有充分接触巴孢子，导致产生抗 PA IgG 抗体，要么接触了炭疽孢子但没有产生抗体。快速、灵敏、准确、精密的抗 PA IgG 抗体测定方法。
Aims:To evaluate potential exposure toBacillis anthracis(Ba) spores in sampling/decontamination workers in the aftermath of an anthrax terror attack.Methods:Fifty six serum samples were obtained from workers involved in environmental sampling forBaspores at the American Media, Inc. (AMI) building in Boca Raton, FL after the anthrax attack there in October 2001. Nineteen sera were drawn from individuals both pre-entry and several weeks after entrance into the building. Nine sera each were drawn from unique individuals at the pre-entry and follow up blood draws. Thirteen donor control sera were also evaluated. Individuals were surveyed forBaexposure by measurement of serumBaanti-protective antigen (PA) specific IgG antibodies using a newly developed fluorescent covalent microsphere immunoassay (FCMIA).Results:Four sera gave positive anti-PA IgG results (defined as anti-PA IgG concentrations ⩾ the mean μg/ml anti-PA IgG from donor control sera (n = 13 plus 2 SD which were also inhibited ⩾ 85% when the serum was pre-adsorbed with PA). The positive sera were the pre-entry and follow up samples of two workers who had received their last dose of anthrax vaccine in 2000.Conclusion:It appears that the sampling/decontamination workers of the present study either had insufficient exposure toBaspores to cause the production of anti-PA IgG antibodies or they were exposed to anthrax spores without producing antibody. The FCMIA appears to be a fast, sensitive, accurate, and precise method for the measurement of anti-PA IgG antibodies.