Forest Bathing Enhances Human Natural Killer Activity and Expression of Anti-Cancer Proteins
Forest Bathing Enhances Human Natural Killer Activity and Expression of Anti-Cancer Proteins
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DOI：
10.1177/03946320070200s202
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发表时间：
2007-04
影响因子：
3.5
通讯作者：
Qing Li;Kanehisa Morimoto;Ari Nakadai;H. Inagaki;M. Katsumata;Takako Shimizu;Y. Hirata;K. Hirata-K.-Hir
中科院分区：
文献类型：
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作者：
Qing Li;Kanehisa Morimoto;Ari Nakadai;H. Inagaki;M. Katsumata;Takako Shimizu;Y. Hirata;K. Hirata-K.-Hir
为探讨森林浴对人体免疫功能的影响，研究了自然杀伤细胞（NK）活性；林场考察期间NK细胞数量及外周血淋巴细胞（PBL）穿孔素、颗粒酶和颗粒蛋白的表达。在知情同意的情况下，从日本东京的三家大公司中选择了12名年龄在37-55岁之间的健康男性受试者。受试者在三个不同的森林田野中经历了三天/两夜的旅行。第一天，受试者在下午在森林田野散步两个小时；第二天，他们上午和下午分别在两个不同的森林田野里走了两个小时。第2、3天采血，测定NK活性；测定PBL中NK细胞、T细胞、颗粒蛋白、穿孔素和颗粒酶A/ b表达细胞的比例。在正常工作日的旅行前进行类似的测量作为对照。几乎所有的受试者（11/12）在旅行后NK活性都比旅行前高（约增加50%）。在旅行前后和第1天和第2天之间NK活性有显著差异。森林浴也显著增加NK、穿孔素、颗粒酶和颗粒酶A/ b表达细胞的数量。综上所述，这些发现表明森林浴之旅可以增加NK细胞的活性，并且这种作用至少部分是通过增加NK细胞的数量和诱导细胞内抗癌蛋白来介导的。
In order to explore the effect of forest bathing on human immune function, we investigated natural killer (NK) activity; the number of NK cells, and perforin, granzymes and granulysin-expression in peripheral blood lymphocytes (PBL) during a visit to forest fields. Twelve healthy male subjects, age 37–55 years, were selected with informed consent from three large companies in Tokyo, Japan. The subjects experienced a three-day/two-night trip in three different forest fields. On the first day, subjects walked for two hours in the afternoon in a forest field; and on the second day, they walked for two hours in the morning and afternoon, respectively, in two different forest fields. Blood was sampled on the second and third days, and NK activity; proportions of NK, T cells, granulysin, perforin, and granzymes A/B-expressing cells in PBL were measured. Similar measurements were made before the trip on a normal working day as the control. Almost all of the subjects (11/12) showed higher NK activity after the trip (about 50% increased) compared with before. There are significant differences both before and after the trip and between days 1 and 2 in NK activity. The forest bathing trip also significantly increased the numbers of NK, perforin, granulysin, and granzymes A/B-expressing cells. Taken together, these findings indicate that a forest bathing trip can increase NK activity, and that this effect at least partially mediated by increasing the number of NK cells and by the induction of intracellular anti-cancer proteins.