Increased secretion of IL-18 in vitro by peripheral blood mononuclear cells of patients with bronchial asthma and atopic dermatitis
Increased secretion of IL-18 in vitro by peripheral blood mononuclear cells of patients with bronchial asthma and atopic dermatitis
复制标题
DOI：
10.1046/j.1365-2249.2001.01664.x
复制
发表时间：
2001-11-01
影响因子：
4.6
通讯作者：
Miike, T
中科院分区：
文献类型：
--
作者：
El-Mezzein, REH;Matsumoto, T;Miike, T
本研究旨在确定IL-18（以前称为IFN-γ诱导因子）是否参与过敏性疾病的发病机制。从过敏性支气管哮喘（BA）、特应性皮炎（AD）患者和无任何过敏性疾病的对照者中获得外周血单个核细胞（PBMC），然后用脂多糖（LPS）或植物血凝素（PHA）培养。用酶免疫分析法测定上清液中IL-18、IFN-γ和IL-13的浓度，用比色微孔板法测定细胞中IFN-γ信使（m）RNA的表达。BA患者（几何平均值（gm）= 189 pg/ml）和AD患者（gm = 172 pg/ml）中的IL-18分泌显著高于非过敏对照（gm = 118 pg/ml）。相反，BA患者（gm = 7.3 IU/ml）和AD患者（gm = 6.8 IU/ml）的IFN-γ分泌显著低于非过敏性对照（gm = 20.7 IU/ml）。在BA患者、AD患者和非过敏对照之间，上清液中IL-13和细胞中IFN-γ mRNA的量没有统计学差异。IL-18可能参与过敏性疾病进行了讨论。
This study was performed to determine whether or not IL-18, formerly called IFN-gamma-inducing factor, is involved in the pathogeneses of allergic disorders. Peripheral blood mononuclear cells (PBMC) were obtained from patients with allergic bronchial asthma (BA), patients with atopic dermatitis (AD) and controls who did not have any allergic disease, and then cultured with lipopolysaccharide (LPS) or phytohaemagglutinin (PHA). The concentrations of IL-18, IFN-gamma and IL-13 in supernatant fluids were determined by enzymatic immunoassaying, and the expression of IFN-gamma messenger (m) RNA in the cells was measured by colorimetric microplate assaying. IL-18 secretion in the BA patients (geometric mean (gm) = 189 pg/ml) and AD patients (gm = 172 pg/ml) was significantly higher than that in non-allergic controls (gm = 118 pg/ml). In contrast, IFN-gamma secretion in the BA patients (gm = 7.3 IU/ml) and AD patients (gm = 6.8 IU/ml) was significantly lower than that in non-allergic controls (gm = 20.7 IU/ml). The amounts of IL-13 in supernatant fluids and IFN-gamma mRNA in cells were not statistically different among the BA patients, AD patients and non-allergic controls. The possible involvement of IL-18 in allergic disorders is discussed.