Visual experience and subsequent sleep induce sequential plastic changes in putative inhibitory and excitatory cortical neurons
Visual experience and subsequent sleep induce sequential plastic changes in putative inhibitory and excitatory cortical neurons
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DOI：
10.1073/pnas.1208093110
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发表时间：
2013-02-19
影响因子：
11.1
通讯作者：
Frank, Marcos G.
中科院分区：
文献类型：
--
作者：
Aton, Sara J.;Broussard, Christopher;Frank, Marcos G.
发育中的初级视皮层的眼优势可塑性由单眼剥夺(MD)启动，并在随后的睡眠中巩固。为了阐明视觉体验和睡眠如何影响神经元的活动和可塑性，我们在清醒的MD和MD后的睡眠期间，连续记录了自由行为猫的颗粒外视觉皮质快峰(FS)中间神经元和可能的主(即兴奋性)神经元。与之前在小鼠身上的报道一致，MD诱导了FS中间神经元的两个相关变化：反应向有利于闭眼的方向转变和抑制放电。对FS中间神经元的睁眼偏向主神经元的棘波时序依赖性抑制可能介导了这些效应。在MD后非快速眼动睡眠期间，主神经元放电增加，对慢波和纺锤波振荡的相位锁定程度更高。眼球优势(OD)向睁眼刺激的转变--只有在MD睡眠后才明显--与MD诱导的FS中间神经元活动的变化以及随后睡眠相关的主神经元活动的变化成正比。在MD后慢波期间，在相邻FS中间神经元后40-300ms放电的主神经元的OD移位最大。基于这些数据，我们认为MD诱导的FS中间神经元的改变在眼优势可塑性中起指导作用，导致睁眼偏向的主神经元之间的去抑制，从而在随后的睡眠中驱动整个视觉皮质的可塑性。
Ocular dominance plasticity in the developing primary visual cortex is initiated by monocular deprivation (MD) and consolidated during subsequent sleep. To clarify how visual experience and sleep affect neuronal activity and plasticity, we continuously recorded extra-granular visual cortex fast-spiking (FS) interneurons and putative principal (i.e., excitatory) neurons in freely behaving cats across periods of waking MD and post-MD sleep. Consistent with previous reports in mice, MD induces two related changes in FS interneurons: a response shift in favor of the closed eye and depression of firing. Spike-timing-dependent depression of open-eye-biased principal neuron inputs to FS interneurons may mediate these effects. During post-MD nonrapid eye movement sleep, principal neuron firing increases and becomes more phase-locked to slow wave and spindle oscillations. Ocular dominance (OD) shifts in favor of open-eye stimulation-evident only after post-MD sleep-are proportional to MD-induced changes in FS interneuron activity and to subsequent sleep-associated changes in principal neuron activity. OD shifts are greatest in principal neurons that fire 40-300 ms after neighboring FS interneurons during post-MD slow waves. Based on these data, we propose that MD-induced changes in FS interneurons play an instructive role in ocular dominance plasticity, causing disinhibition among open-eye-biased principal neurons, which drive plasticity throughout the visual cortex during subsequent sleep.