POU-domain proteins Pit-1 and Oct-1 interact to form a heteromeric complex and can cooperate to induce expression of the prolactin promoter.
POU-domain proteins Pit-1 and Oct-1 interact to form a heteromeric complex and can cooperate to induce expression of the prolactin promoter.
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DOI：
10.1101/gad.5.7.1309
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发表时间：
1991-07
影响因子：
10.5
通讯作者：
J. Voss;L. Wilson;Michael G. Rosenfeld
中科院分区：
文献类型：
--
作者：
J. Voss;L. Wilson;Michael G. Rosenfeld
POU结构域家族的转录激活因子Pit-1和Oct-1在垂体前叶细胞中共表达。我们证明，垂体特异性发育调节Pit-1可以作为异源二聚体与广泛表达的100 kD Oct-1蛋白结合到大鼠催乳素基因中的关键组织特异性顺式活性元件。Pit-1和Oct-1也可以在没有DNA的情况下通过它们的POU结构域缔合。Pit-1和Oct-1在异源细胞类型中的共表达导致在天然催乳素启动子或单个Pit-1响应元件的控制下对基因的可再现的2至2.5倍的协同转录效应。这些结果表明，POU结构域基因家族的不同成员之间的异二聚体和同二聚体相互作用的组合模式，可以导致当这个大家族的成员在发育和建立的器官系统的细胞中共表达时，可能调节差异发育基因激活。
Two members of the POU-domain family of transcriptional activators, Pit-1 and Oct-1, are coexpressed in cells of the anterior pituitary gland. We demonstrate that the pituitary-specific developmental regulator Pit-1 can bind as a heterodimer with the widely expressed 100-kD Oct-1 protein to critical tissue-specific cis-active elements in the rat prolactin gene. Pit-1 and Oct-1 can also associate, in the absence of DNA, via their POU domains. Coexpression of Pit-1 and Oct-1 in a heterologous cell type results in reproducible 2- to 2.5-fold synergistic transcriptional effects on genes under control of the native prolactin promoter or of a single Pit-1 response element. These results suggest that a combinatorial pattern of heterodimeric and homodimeric interactions between different members of the POU-domain gene family can result when members of this large family are coexpressed in cells of developing and established organ systems, potentially regulating differential developmental gene activation.