Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion
Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion
复制标题
RalB参与香叶基香叶基转移酶I对胶质瘤细胞迁移和侵袭的影响
DOI：
10.1007/s12094-014-1263-x
复制
发表时间：
2015
影响因子：
3.4
通讯作者：
Yu R.
中科院分区：
文献类型：
--
作者：
Song X.;Hua L.;Xu Y.;Fang Z.;Wang Y.;Gao J.;Shi Q.;Zhou X.;Yu R.
目的探讨香叶基香叶基转移酶I（geranylgeranyltransferase I，GGT-I）在胶质瘤细胞迁移和侵袭中的作用及其可能机制方法采用创伤愈合实验和transwell实验研究GGT-I和Ras样GTP酶B（Ral B）对胶质瘤细胞迁移和侵袭的影响结果发现敲低GGT-I或Ral B均能显著降低胶质瘤细胞的迁移和侵袭能力。GGTase-I下调抑制RalB膜结合。结论RalB可能是促进GGT-Ⅰ诱导的恶性胶质瘤侵袭表型的作用靶点之一。
PurposeThe present study aimed at investigating the role and potential mechanism of geranylgeranyltransferase I (GGTase-I) on glioma cell migration and invasion.MethodsWound-healing assay and transwell assay were performed to study whether GGTase-I and Ras-like GTPase B (RalB) have effect on glioma cell migration and invasion.ResultsWe found that knockdown of GGTase-I or RalB both significantly decreased the migratory and invasive abilities of glioma cells. GGTase-I down-regulation suppressed RalB membrane association. Moreover, down-regulation of RalB partially abolished the effect of GGTβ over-expression-induced glioma cell migration and invasion increase.ConclusionsThese findings suggest that RalB might be one of the targets for facilitating the invasive phenotype of malignant gliomas induced by GGTase-I.