Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation
Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation
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DOI：
10.3390/ijms20071691
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发表时间：
2019-04-04
影响因子：
5.6
通讯作者：
Zeng, Fanchang
中科院分区：
文献类型：
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作者：
Guo, Haixia;Guo, Huihui;Zeng, Fanchang
植物体细胞胚胎发生（somatic embryogenesis，SE）过程是植物对激素非生物胁迫的典型反应之一，通过不同蛋白质的动态表达，构成复杂的生物活性调控网络，促进植物的全能性。植物体胚发育包括两个关键阶段：初生胚性愈伤组织再分化和体细胞胚发育起始，这导致全能性。利用同量异序标记串联质量标签（TMT）大规模定量蛋白质组学技术，研究了棉花非胚性愈伤组织（NEC）、初生胚性愈伤组织（PEC）和球形胚（GE）蛋白质表达的动态变化。共鉴定了9369种蛋白质（6730种定量）;在PEC与NEC、GE与PEC和GE与NEC中分别鉴定了805、295和1242种差异累积蛋白质（DAP）。共鉴定出805个差异蛋白，其中309个在PEC中表达上调，496个表达下调。在GE和PEC之间鉴定的295个DAP中，分别有174个和121个蛋白质上调和下调。1242差异丰富的蛋白质，584和658蛋白上调和下调，分别在GE与NEC。我们还补充了蛋白质组学分析的真实性和准确性。系统分析表明，过氧化物酶、光合作用、环境胁迫响应过程、氮代谢、激素响应/信号转导、转录/转录后修饰等都参与了体细胞胚胎发生过程。本研究结果为进一步研究棉花全能性过程中DAP在SE转分化过程中的作用提供了蛋白质组学分子基础。
The somatic embryogenesis (SE) process of plants, as one of the typical responses to abiotic stresses with hormone, occurs through the dynamic expression of different proteins that constitute a complex regulatory network in biological activities and promotes plant totipotency. Plant SE includes two critical stages: primary embryogenic calli redifferentiation and somatic embryos development initiation, which leads to totipotency. The isobaric labels tandem mass tags (TMT) large-scale and quantitative proteomics technique was used to identify the dynamic protein expression changes in nonembryogenic calli (NEC), primary embryogenic calli (PEC) and globular embryos (GEs) of cotton. A total of 9369 proteins (6730 quantified) were identified; 805, 295 and 1242 differentially accumulated proteins (DAPs) were identified in PEC versus NEC, GEs versus PEC and GEs versus NEC, respectively. Eight hundred and five differentially abundant proteins were identified, 309 of which were upregulated and 496 down regulated in PEC compared with NEC. Of the 295 DAPs identified between GEs and PEC, 174 and 121 proteins were up- and down regulated, respectively. Of 1242 differentially abundant proteins, 584 and 658 proteins were up- and down regulated, respectively, in GEs versus NEC. We have also complemented the authenticity and accuracy of the proteomic analysis. Systematic analysis indicated that peroxidase, photosynthesis, environment stresses response processes, nitrogen metabolism, phytohormone response/signal transduction, transcription/posttranscription and modification were involved in somatic embryogenesis. The results generated in this study demonstrate a proteomic molecular basis and provide a valuable foundation for further investigation of the roles of DAPs in the process of SE transdifferentiation during cotton totipotency.