Cyclic nucleotide-gated ion channel 6 is involved in extracellular ATP signaling and plant immunity.
Cyclic nucleotide-gated ion channel 6 is involved in extracellular ATP signaling and plant immunity.
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DOI：
10.1111/tpj.15636
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发表时间：
2022-03
期刊：
影响因子：
7.2
通讯作者：
Stacey, Gary
中科院分区：
文献类型：
--
作者：
Duong, Ha N.;Cho, Sung-Hwan;Wang, Limin;Pham, An Q.;Davies, Julia M.;Stacey, Gary
细胞外ATP（eATP）是植物和动物的危险信号。在植物中，eATP由质膜（PM）定位的受体P2 K1（LecRK-I.9）识别。对eATP添加的第一个可测量的反应是细胞质游离钙水平（[Ca 2 +]cyt）的快速上升，这需要P2 K1。然而，介导[Ca 2 +]cyt升高的特定转运蛋白/通道蛋白尚不清楚。通过正向遗传筛选，我们确定了一个拟南芥甲基磺酸乙酯（EMS）突变体受损的[Ca 2 +]cyt响应eATP。定位克隆显示，该突变位于cngc 6基因，该基因编码环核苷酸门控离子通道6（CNGC 6）。CNGC 6基因的突变导致响应eATP的PM内向Ca 2+电流显著降低。eATP诱导的丝裂原活化蛋白激酶激活和基因表达也显着降低cngc 6突变体植物。此外，cngc 6突变体植物也更容易受到细菌病原体假单胞菌。综上所述，我们的研究结果表明CNGC 6在介导eATP诱导的[Ca 2 +]cyt信号传导以及植物免疫中起着至关重要的作用。
Extracellular ATP (eATP) is known to act as a danger signal in both plants and animals. In plants, eATP is recognized by the plasma membrane (PM)-localized receptor P2K1 (LecRK-I.9). Among the first measurable responses to eATP addition is a rapid rise in cytoplasmic free calcium levels ([Ca2+]cyt), which requires P2K1. However, the specific transporter/channel proteins that mediate this rise in [Ca2+]cyt are unknown. Through a forward genetic screen, we identified an Arabidopsis ethylmethanesulfonate (EMS) mutant impaired in the [Ca2+]cyt response to eATP. Positional cloning revealed that the mutation resided in the cngc6 gene, which encodes cyclic nucleotide-gated ion channel 6 (CNGC6). Mutation of the CNGC6 gene led to a notable decrease in the PM inward Ca2+ current in response to eATP. eATP-induced mitogen-activated protein kinase activation and gene expression were also significantly lower in cngc6 mutant plants. In addition, cngc6 mutant plants were also more susceptible to the bacterial pathogen Pseudomonas syringae. Taken together, our results indicate that CNGC6 plays a crucial role in mediating eATP-induced [Ca2+]cyt signaling, as well as plant immunity.
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