Preparation of Crystalline Samples of Amyloid Fibrils and Oligomers.
Preparation of Crystalline Samples of Amyloid Fibrils and Oligomers.
复制标题
淀粉样原纤维和寡聚物晶体样品的制备。
DOI：
10.1007/978-1-4939-2978-8_13
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Eisenberg,David
中科院分区：
文献类型：
--
作者：
Moshe,Asher;Landau,Meytal;Eisenberg,David
淀粉样纤维和低聚物的分子结构是理解和控制其形成所必需的。然而，它们的部分无序和多态性阻碍了结构分析。幸运的是，来自淀粉样蛋白的短片段，其表现出与全长蛋白质相似的生物物理性质，也形成原纤维和寡聚体，并且它们的原子结构可以被确定。在这里，我们描述的实验程序，用于评估纤维形成能力的淀粉样肽段和它们的结晶。
The molecular structures of amyloid fibers and oligomers are required in order to understand and control their formation. Yet, their partially disordered and polymorphic nature hinders structural analyses. Fortunately, short segments from amyloid proteins, which exhibit similar biophysical properties to the full-length proteins, also form fibrils and oligomers and their atomic structures can be determined. Here we describe experimental procedures used to assess fiber-forming capabilities of amyloid peptide segments and their crystallization.