Characterization of Gastric Microbiota in Twins
Characterization of Gastric Microbiota in Twins
复制标题
双胞胎胃微生物群的特征。
DOI：
10.1007/s00284-016-1176-8
复制
发表时间：
2017-02-01
影响因子：
2.6
通讯作者：
Xuan, Shiying
中科院分区：
文献类型：
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作者：
Dong, Quanjiang;Xin, Yongning;Xuan, Shiying
宿主遗传背景在胃微生物群发育中的作用尚未明确。本研究旨在了解双胞胎胃微生物群的生物多样性、结构和组成。共有四对双胞胎和八名没有血缘关系的个体参加了这项研究。内镜检查时进行胃窦活检。扩增细菌16S rRNA基因并进行焦磷酸测序。序列分析了胃微生物群的组成、结构和α和β多样性。变形菌门、厚壁菌门、拟杆菌门、放线菌门和梭杆菌门是胃微生物群中最主要的门。每一个个体，无论是双胞胎还是不相关的个体，都拥有不同组成的微生物群。没有证据表明，同卵双胞胎的胃微生物群的丰富度和均匀度与非亲属相比有额外的相似性。计算theta（YC）和PCoA表明，与非亲缘个体相比，双胞胎之间的胃微生物群落结构相似性没有增加。相比之下，幽门螺杆菌感染使微生物群结构发生了巨大的变化。这些结果表明，宿主遗传背景对胃微生物群的形成影响不大。胃微生物群的这一特性有助于从遗传角度研究微生物群在发病机制中的作用。
Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. Antral biopsies were obtained during endoscopy. The bacterial 16S rRNA gene was amplified and pyrosequenced. Sequences were analyzed for the composition, structure, and alpha and beta diversities of gastric microbiota. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria were the most predominant phyla of gastric microbiota. Each individual, twins as well as unrelated individuals, harbored a microbiota of distinct composition. There was no evidence of additional similarity in the richness and evenness of gastric microbiota among co-twins as compared to unrelated individuals. Calculations of theta(YC) and PCoA demonstrated that the structure similarity of gastric microbial community between co-twins did not increase compared to unrelated individuals. In contrast, the structure of microbiota was altered enormously by Helicobacter pylori infection. These results suggest that host genetic backgrounds had little effect in shaping the gastric microbiota. This property of gastric microbiota could facilitate the studies discerning the role of microbiota from genetic grounds in the pathogenesis.