Demonstration of Thy-1 antigen on pluripotent hemopoietic stem cells in the rat.
Demonstration of Thy-1 antigen on pluripotent hemopoietic stem cells in the rat.
复制标题
大鼠中多能血态干细胞上的Thy-1抗原的证明。
DOI：
10.1084/jem.148.5.1351
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发表时间：
1978-11-01
期刊：
影响因子：
--
通讯作者：
Morris RJ
中科院分区：
文献类型：
--
作者：
Goldschneider I;Gordon LK;Morris RJ
使用三种方法证明大鼠多能造血干细胞表面存在 Thy-1 抗原。首先，通过与 Thy-1 抗原抗体一起孵育，可防止来自胎儿肝脏、新生儿脾脏和成人骨髓的干细胞在受辐射的受者脾脏中形成造血集落（集落形成单位测定）。针对纯化的大鼠脑 Thy-1 抗原的高度特异性兔异种抗血清和针对 Thy-1.1 阳性胸腺细胞的小鼠同种抗血清同样有效。这种抑制作用被纯化的 Thy-1 抗原中和。在第二个系列的实验中，通过 FACS 分离了 Thy-1 阳性和 Thy-1 阴性的有核骨髓细胞群。 Thy-1 阳性群体的所有造血干细胞活性均得到恢复。这些干细胞是 Thy-1 抗原最强阳性的细胞之一，相对荧光强度位于前 25 个百分位数。第三系列实验显示了 Thy-1 抗原与大鼠骨髓淋巴细胞抗原 (BMLA) 的关系。兔抗 BMLA 血清是针对淋巴细胞样骨髓细胞的空群而产生的，已被证明具有抗干细胞活性。在这里，我们通过双重免疫荧光、共帽化和差异吸收研究证明 Thy-1 和 BMLA 是同一分子的一部分。
Three approaches were used to demonstrate the presence of Thy-1 antigen on the surface of pluripotent hemopoietic stem cells in the rat. In the first, stem cells from fetal liver, neonatal spleen, and adult bone marrow were prevented from forming hemopoietic colonies in the spleens of irradiated recipients spleen (colony-forming unit assay) by incubation with antibodies to Thy-1 antigen. Highly specific rabbit heteroantiserum to purified rat brain Thy-1 antigen and mouse alloantisera to Thy-1.1-positive thymocytes were equally effective. This inhibition was neutralized by purified Thy-1 antigen. In a second series of experiments, Thy-1-positive and Thy-1-negative populations of nucleated bone marrow cells were separated by the FACS. All of the hemopoietic stem cell activity was recovered in the Thy-1-positive population. The stem cells were among the most strongly positive for Thy-1 antigen, being in the upper 25th percentile for relative fluorescence intensity. The relationships of Thy-1 antigen to the rat bone marrow lymphocyte antigen (BMLA) was shown in a third series of experiments. Rabbit anti-BMLA serum, which is raised against a null population of lymphocyte-like bone marrow cells, has been shown to have anti-stem cell activity. Here we demonstrate by double immunofluorescence, cocapping, and differential absorption studies that Thy-1 and BMLA are parts of the same molecule.