Kinetic determination of focal adhesion protein formation
Kinetic determination of focal adhesion protein formation
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DOI：
10.1006/bbrc.2000.2653
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发表时间：
2000-05-10
影响因子：
3.1
通讯作者：
Goldmann, WH
中科院分区：
文献类型：
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作者：
Goldmann, WH
我检查了整合素（α（IIb）β（3））和纯化的粘着斑蛋白，包括α-辅肌动蛋白，细丝蛋白，黏着斑蛋白，塔林蛋白和F-肌动蛋白之间的结合动力学。使用静态光散射技术，我观察到的顺序talin >细丝蛋白> F-肌动蛋白> α-辅肌动蛋白>（talin时结合到黏着斑蛋白）的亲和力较低时，整合素与纤连蛋白复合。未检测到整合素和黏着斑蛋白之间结合。计算的解离常数（Kd）在0.4 μ M和5 μ M之间。这些结果部分证实了以前使用不同方法发表的数据。粘着斑蛋白在体外相互作用的适度亲和力可能指示细胞，血小板，获得高度的通用性和速度。(C)北京大学出版社.
I examined the binding kinetics between integrin (alpha(IIb)beta(3)) and purified focal adhesion proteins, including alpha-actinin, filamin, vinculin, talin, and F-actin. Using static light-scatter technique, I observed affinities of the order talin > filamin > F-actin > alpha-actinin > (talin when bound to vinculin) which were lower when integrin was complexed with fibronectin. No binding between integrin and vinculin was detected. The calculated dissociation constants (K-d) ranged between 0.4 mu M and 5 mu M. These results in part confirm previously published data using different methods. The modest affinity with which the focal adhesion proteins interact in vitro might be indicative of how cells, e.g., thrombocytes, gain a high degree of versatility and velocity. (C) 2000 Academic Press.