Pretreatment of wheat straw with ligninolytic fungi for increased biogas productivity
Pretreatment of wheat straw with ligninolytic fungi for increased biogas productivity
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DOI：
10.1007/s13762-019-02277-8
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发表时间：
2019-11
影响因子：
3.1
通讯作者：
T. Shah;R. Ullah
中科院分区：
文献类型：
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作者：
T. Shah;R. Ullah
利用农业废弃生物质生产沼气是管理未来能源需求的可持续方法。为此，需要进行预处理以去除农业生物质中的木质素，使其成为发酵生物体易于消化的底物。目前的研究是为了从自然栖息地筛选最有效的木质素降解真菌培养物。用强效木质素分解真菌进行预处理可能是一种有效且经济的降解木质素的方法。木质纤维素生物质中木质素的存在是降低沼气产量的限速因素。因此，根据最大的Azure B染料脱色能力来选择最佳的真菌菌株。这些菌株能够积极产生木质素过氧化物酶和漆酶，并在分批培养的 7 天内减少了麦秆 (WS) 中 48.2% 的木质素。扫描电子显微照片显示，仅在经过真菌处理的麦秆中可见降解。与未处理的 WS 样品相比，木质素真菌培养物预处理 WS 的沼气产量提高了五倍。值得注意的是，这些快速作用的木质素降解真菌在较短的预处理时间内产生了沼气，并且还减少了厌氧消化过程中的滞后阶段。这些发现表明，用天然木质素分解真菌进行预处理可以被认为是一种较便宜的方法。该处理还可有效提高木质纤维素生物质的消化率，并适用于沼气生产。
Biogas production from the agriculture waste biomass is a sustainable approach to managing the energy demand in the future. To do so, pretreatment is needed to remove lignin from the agribiomass to make it easily digestible substrate for the fermentative organisms. The current study was carried out to screen the most effective lignin-degrading fungal culture from their natural habitat. Pretreatment with robust ligninolytic fungi could be an efficient and economical method to degrade lignin. The presence of lignin in lignocellulosic biomass is the rate-limiting factor which reduces the biogas production. So, the best fungal strains were selected based on maximal Azure B dye decolorization. The strains were actively producing lignin peroxidases and laccases and decreased 48.2% of lignin from the wheat straw (WS) within 7 days of batch incubation. The scanning electron micrograph demonstrated visible degradation only in the fungi-treated wheat straw. The ligninolytic fungal culture pretreatment of WS enhanced the biogas yield five times compared with the untreated WS sample. Notably, these fast-acting lignin-degrading fungi gave a biogas yield with a short pretreatment time and additionally reduced the lag phase during the anaerobic digestion process. These findings showed that pretreatment with natural ligninolytic fungi could be considered as a less expensive method. The treatment is also effective in increasing the digestibility of lignocellulosic biomass and suitable for biogas production.