Human Periosteum-Derived Cells Combined With Superporous Hydroxyapatite Blocks Used as an Osteogenic Bone Substitute for Periodontal Regenerative Therapy: An Animal Implantation Study Using Nude Mice
Human Periosteum-Derived Cells Combined With Superporous Hydroxyapatite Blocks Used as an Osteogenic Bone Substitute for Periodontal Regenerative Therapy: An Animal Implantation Study Using Nude Mice
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DOI：
10.1902/jop.2009.090523
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发表时间：
2010-03-01
影响因子：
4.3
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
--
作者：
Kawase, Tomoyuki;Okuda, Kazuhiro;Yoshie, Hiromasa
背景资料：超多孔（85%）羟基磷灰石（HA）块最近开发，以提高骨传导性，但它往往是不成功的临床上用于治疗牙周骨缺损。本研究的主要目的是开发一种临床上适用的组织工程骨替代品，使用这种HA块和人牙槽骨膜衍生cells.Methods：市售的超多孔HA块进行酸处理，并进行三维（3D）培养骨膜细胞的培养。通过扫描电子显微镜在断裂表面上观察处理的HA块的孔区域中的细胞。成骨诱导后，将细胞HA复合物植入裸鼠皮下。结果：酸处理使骨孔间的相互连接增大，骨膜细胞向骨孔内渗透。在这些条件下，细胞维持>2周，在HA块的深孔区域中没有明显的细胞死亡。细胞HA复合物，接受体外成骨诱导形成类骨质在孔区域的处理后的HA块在体内。相比之下，在非预处理的，无细胞的HA块，在体内评估的大多数孔隙区域保持cellfree.Conclusions：我们的研究结果表明，酸处理的HA块可以作为一个更好的支架，为3D高密度培养人骨膜细胞在体外，这种细胞HA复合物有显着的成骨潜力在体内植入的网站。与无细胞HA块相比，我们的细胞HA复合物使用骨膜细胞，这是最容易为临床牙周病学家，显示出有希望的结果作为牙周再生治疗的骨替代物。期刊杂志2010;81：420-427。
Background: A superporous (85%) hydroxyapatite (HA) block was recently developed to improve osteoconductivity, but it was often not clinically successful when used to treat periodontal osseous defects. The primary purpose of this study is to develop a clinically applicable tissue-engineered bone substitute using this HA block and human alveolar periosteum-derived cells.Methods: Commercially available superporous HA blocks were acid treated and subjected to a three-dimensional (3D) culture for periosteal cell cultivation. Cells in the pore regions of the treated HA block were observed on the fracture surface by scanning electron microscopy. After osteogenic induction, the cell HA complexes were implanted subcutaneously in nude mice. Osteoid formation was histologically evaluated.Results: Acid treatment enlarged the interconnections among pores, resulting in the deep penetration of periosteal cells. Under these conditions, cells were maintained for >2 weeks without appreciable cell death in the deep pore regions of the HA block. The cell HA complexes that received in vitro osteogenic induction formed osteoids in pore regions of the treated HA blocks in vivo. In contrast, most pore regions in the non-pretreated, cell-free HA blocks that were evaluated in vivo remained cell free.Conclusions: Our findings suggest that an acid-treated HA block could function as a better scaffold for the 3D high-density culture of human periosteal cells in vitro, and this cell HA complex had significant osteogenic potential at the site of implantation in vivo. Compared with the cell-free HA block, our cell HA complex using periosteal cells, which are the most accessible for clinical periodontists, showed promising results as a bone substitute in periodontal regenerative therapy. J Periodontal 2010;81:420-427.