A cis‐encoded sRNA controls the expression of fabH2 in Yersinia
A cis‐encoded sRNA controls the expression of fabH2 in Yersinia
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DOI：
10.1016/j.febslet.2014.04.005
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发表时间：
2014-05
期刊：
影响因子：
3.5
通讯作者：
Pei Lu;Yong Zhang;Yangbo Hu;M. Francis;Shiyun Chen
中科院分区：
文献类型：
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作者：
Pei Lu;Yong Zhang;Yangbo Hu;M. Francis;Shiyun Chen
YsrH是一种新的顺式编码的sRNA，位于fabH2的相对链上，fabH2是细菌脂肪酸生物合成所必需的。在本研究中，YsrH介导的调控abH 2表达在假结核耶尔森氏菌进行了研究。YsrH的组成性和诱导性过表达降低了abH2的mRNA水平，而下游fabD和fabG的表达不受影响。多核苷酸磷酸化酶（PNIPs）也发挥了重要作用，在这一调控过程中，介导的YsrH衰减的指数阶段。因此，我们的数据定义了acis编码的sRNA，其通过也涉及PNTR的调节机制调节脂肪酸合成。
YsrH is a novelcis-encoded sRNA located on the opposite strand tofabH2, which is essential for fatty acid biosynthesis in bacteria. In this study, YsrH-mediated regulation offabH2expression was investigated inYersinia pseudotuberculosis. Constitutive and inducible over-expression of YsrH decreased the mRNA level offabH2, while expression of downstreamfabDandfabGremained unaffected. Polynucleotide phosphorylase (PNPase) also played an important role in this regulation process by mediating YsrH decay in the exponential phase. Thus, our data defines acis-encoded sRNA that regulates fatty acid synthesis via a regulatory mechanism also involving PNPase.