Shigella Pathogenesis Modeling with Tissue Culture Assays.
Shigella Pathogenesis Modeling with Tissue Culture Assays.
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DOI：
10.1002/cpmc.57
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发表时间：
2018-08
影响因子：
--
通讯作者：
Payne SM
中科院分区：
文献类型：
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作者：
Koestler BJ;Ward CM;Payne SM
志贺菌属是一种肠侵入性的人类病原体，其感染结肠上皮并引起志贺菌病，一种传染性结肠炎疾病。没有疫苗可用于预防或治疗志贺氏菌病，志贺氏菌的抗生素耐药菌株正在增加，强调需要更深入地了解志贺氏菌的发病机制，以设计有效的抗菌治疗。小动物模型不能再现志贺氏菌病，因此组织培养细胞可作为研究志贺氏菌致病机制的模型系统。在这里，我们描述的协议，列举志贺氏菌的入侵，细胞-细胞传播，和斑块形成的组织培养细胞系Henle-407和CoN-841。此外，我们描述了一种新的方法来研究志贺菌入侵的主要肠类。这些方案可用于检测志贺菌毒力的不同方面。
Shigella is an enteroinvasive human pathogen that infects the colonic epithelium and causes Shigellosis, an infectious diarrheal disease. There is no vaccine for the prevention or treatment of Shigellosis and antibiotic resistant strains of Shigella are increasing, emphasizing the need for deeper understanding of Shigella pathogenesis in order to design effective antimicrobial therapies. Small animal models do not recapitulate Shigellosis, therefore tissue cultured cells have served as model systems to study Shigella pathogenesis. Here, we describe protocols to enumerate Shigella invasion, cell-cell spread, and plaque formation in the tissue cultured cell lines Henle-407 and CoN-841. Additionally, we describe a new method to study Shigella invasion in primary intestinal enteroids. These protocols can be used to examine different aspects of Shigella virulence.