Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes
Functional metagenomic analysis reveals rivers are a reservoir for diverse antibiotic resistance genes
复制标题
DOI：
10.1016/j.vetmic.2014.02.017
复制
发表时间：
2014-07-16
影响因子：
3.3
通讯作者：
Wellington, E. M.
中科院分区：
文献类型：
--
作者：
Amos, G. C. A.;Zhang, L.;Wellington, E. M.
环境中含有大量未培养的细菌，是新的和现存的耐药基因的潜在来源，这些耐药基因可能与传播到环境水库中的临床重要细菌重组。有证据表明，由于适应基因在移动的元素上的聚集，污染可以选择抗性。本研究的目的是建立废水处理厂（WWTP）的污水处理到河流的影响，通过使用文化独立的方法来研究的WWTP下游的抗性基因的多样性相比，上游。在大肠杆菌中构建宏基因组文库，并筛选对阿米卡星、庆大霉素、新霉素、氨苄青霉素和环丙沙星的表型抗性。利用转座子诱变技术鉴定抗性基因。在宏基因组文库中回收的表型抗性克隆的数量中观察到WWTP下游的显著增加。回收了常见的β-内酰胺酶，如bla（TEM），以及各种乙酰转移酶和不寻常的转运蛋白基因，有证据表明新出现的耐药机制。预测的蛋白质与已知序列的相似性表明基因起源于非常多样化的细菌。这项研究表明，废水处理增加了水库的阻力机制，在环境中，无论是通过添加抗性基因或输入的代理选择性耐药表型。(C)2014作者由爱思唯尔公司出版
The environment harbours a significant diversity of uncultured bacteria and a potential source of novel and extant resistance genes which may recombine with clinically important bacteria disseminated into environmental reservoirs. There is evidence that pollution can select for resistance due to the aggregation of adaptive genes on mobile elements. The aim of this study was to establish the impact of waste water treatment plant (WWTP) effluent disposal to a river by using culture independent methods to study diversity of resistance genes downstream of the WWTP in comparison to upstream. Metagenomic libraries were constructed in Escherichia coli and screened for phenotypic resistance to amikacin, gentamicin, neomycin, ampicillin and ciprofloxacin. Resistance genes were identified by using transposon mutagenesis. A significant increase downstream of the WWTP was observed in the number of phenotypic resistant clones recovered in metagenomic libraries. Common beta-lactamases such as bla(TEM) were recovered as well as a diverse range of acetyltransferases and unusual transporter genes, with evidence for newly emerging resistance mechanisms. The similarities of the predicted proteins to known sequences suggested origins of genes from a very diverse range of bacteria. The study suggests that waste water disposal increases the reservoir of resistance mechanisms in the environment either by addition of resistance genes or by input of agents selective for resistant phenotypes. (C) 2014 The Authors. Published by Elsevier B.V.